Optional Method: Remove and Re-Introduce Muscle Electrical Activity
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Results: Accurate and Efficient Estimation of the Muscle Volume of Live Zebrafish Larvae at Selected Stages of Activity
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Conclusion
副本
Our protocol allows us to study how tissues, such as skeletal muscle, regulate their size, which remains a key question in cell and developmental biology with important physiological and medical implications. The main advantage of our technique is
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Optical clarity is a major advantage for cell biological and physiological work in zebrafish. Robust methods for measurement of cell growth in individual animals are described that permit novel insights into how growth of skeletal muscle and neighboring tissues are integrated with whole body growth.