This research aims to highlight the dynamism and broad application of Langendorff perfusion as a research tool and its immense utility in cardiovascular disease research. It also highlights the importance of tailoring the different perfusion parameters, such as flows, pressures, perfusate, and temperature to better fit experimental needs. This protocol offers an alternative approach to the current status quo of Langendorff perfusion.
It suggests that using lower than normal perfusion pressures such as 30 to 35 millimeters of mercury instead of the usual 60 to 80 millimeters of mercury could be beneficial for certain types of experiments and could improve overall scientific outcomes of relevant studies. This protocol enhances the significance of Langendorff perfusion as a technique for the cardiovascular research by eliminating the loss of function that is dependent on the technique. Furthermore, it is expected to start a conversation about the best perfusion practices for clinically perfused cardiac grafts, such as those that are being preserved for transplantation.
