<meta charset="utf8"></head><body>通过生长因子优化提高微藻中叶绿素的产生

<ol>
	<li>Khan, M. I., Shin, J. H., Kim, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+promising+future+of+microalgae:+Current+status,+challenges,+and+optimization+of+a+sustainable+and+renewable+industry+for+biofuels,+feed,+and+other+products.">The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products.</a> Microb Cell Fact. 17 (1), 36 (2018).</li><li>Otero-Paternina, A. S. M., Cruz-Casallas, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+the+hydrocarbon+phenanthrene+on+Chlorella+vulgaris+(Chlorellaceae)+growth.">Effect of the hydrocarbon phenanthrene on Chlorella vulgaris (Chlorellaceae) growth.</a> Acta Biol Col. 18 (1), 87-98 (2013).</li><li>Pancha, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Salinity-induced+oxidative+stress+enhanced+the+biofuel+production+potential+of+microalgae+Scenedesmus+sp.+CCNM+1077.">Salinity-induced oxidative stress enhanced the biofuel production potential of microalgae Scenedesmus sp. CCNM 1077.</a> Bioresour Techno. 189, 341-348 (2015).</li><li>Ortiz, M. L., Cort&#233;s, C. E., S&#225;nchez, J., Padilla, J., Otero, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluating+microalgae+Chlorella+sorokiniana+growth+in+different+culture+mediums+in+autotrophic+and+mixotrophic+conditions.">Evaluating microalgae Chlorella sorokiniana growth in different culture mediums in autotrophic and mixotrophic conditions.</a> Orinoquia. 16 (1), 11-20 (2012).</li><li>Camacho Kurmen, J. E., Gonz&#225;lez, G., Klotz, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Astaxanthin+production+in+Haematococcus+pluvialis+under+different+stress+conditions.">Astaxanthin production in Haematococcus pluvialis under different stress conditions.</a> Nova. 11 (19), 94-104 (2013).</li><li>. Production of chlorophyll and astaxanthin from Haematococcus pluvialis under nitrogen deficiency stress in a 5-liter Biostat Aplus bioreactor Available from: <a target="_blank" href="https://repositorio.unicolmayor.edu.co/handle/unicolmayor/2735">https://repositorio.unicolmayor.edu.co/handle/unicolmayor/2735</a> (2019)</li><li>Bischoff, H. W., Bold, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phycological+studies+IV.+Some+soil+algae+from+Enchanted+Rock+and+related+algal+species.">Phycological studies IV. Some soil algae from Enchanted Rock and related algal species.</a> University of Texas. 6318, 95 (1963).</li><li>Cerezo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=LED+characterization+through+the+use+and+programming+of+a+spectrometer.">LED characterization through the use and programming of a spectrometer.</a> Universidad Polit&#233;cnica de. 22323, (2013).</li><li>Couso, I., Vila, M., Vigara, J., Cordero, B. F., Vargas, M. &. #. 1. 9. 3. ;. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+of+carotenoids+and+regulation+of+the+carotenoid+biosynthesis+pathway+in+response+to+high+light+stress+in+the+unicellular+microalga+Chlamydomonas+reinhardtii.">Synthesis of carotenoids and regulation of the carotenoid biosynthesis pathway in response to high light stress in the unicellular microalga Chlamydomonas reinhardtii.</a> Eur J Phycol. 47 (3), 223-232 (2012).</li><li>Vera-L&#243;pez, F., Mart&#237;nez, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pigments+in+microalgae:+Functions,+applications,+and+overproduction+techniques.">Pigments in microalgae: Functions, applications, and overproduction techniques.</a> BioTechnology. 25 (5), 35-51 (2021).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>C3H6O</td>
			<td>Meyer</td>
			<td>67-64-1</td>
			<td>Acetone 90%</td>
		</tr>
		<tr>
			<td>15 mL tube</td>
			<td>Biologix</td>
			<td>10-9502</td>
			<td>Test tube</td>
		</tr>
		<tr>
			<td>2510-DTH</td>
			<td>Branson</td>
			<td>D-73595</td>
			<td>Sonicator</td>
		</tr>
		<tr>
			<td>5 mL screw cap test tube</td>
			<td>Kimax</td>
			<td>45066-13100</td>
			<td>Test tube</td>
		</tr>
		<tr>
			<td>50 mL centrifuge tube</td>
			<td>Biologix</td>
			<td>10-9151</td>
			<td>Test tube</td>
		</tr>
		<tr>
			<td>Aluminum foil</td>
			<td>Reynolds</td>
			<td>611 standard, 12&#34; x 1000 feet</td>
			<td>Test tube cover&#160;</td>
		</tr>
		<tr>
			<td>CaCl2</td>
			<td>Meyer</td>
			<td>0925-250</td>
			<td>Calcium Chloride</td>
		</tr>
		<tr>
			<td>Centrifuge&#160;</td>
			<td>Dynamica</td>
			<td>14 R</td>
			<td>Centrifuge Refrigerated</td>
		</tr>
		<tr>
			<td>CoCl2</td>
			<td>Merck</td>
			<td>1057-100</td>
			<td>Cobalt dichloride</td>
		</tr>
		<tr>
			<td>FeCl3</td>
			<td>Merck</td>
			<td>157740</td>
			<td>Iron(III) Chloride</td>
		</tr>
		<tr>
			<td>K2HPO4</td>
			<td>Meyer</td>
			<td>2051-250</td>
			<td>Dipotassium Phosphate</td>
		</tr>
		<tr>
			<td>KH2PO4</td>
			<td>Meyer</td>
			<td>2055-250</td>
			<td>Monopotassium Phosphate</td>
		</tr>
		<tr>
			<td>MgSO4</td>
			<td>Meyer</td>
			<td>1605-250</td>
			<td>Magnesium Sulphate</td>
		</tr>
		<tr>
			<td>Micropipette</td>
			<td>LabNet</td>
			<td>Model Beta-Pette</td>
			<td>Micropipette</td>
		</tr>
		<tr>
			<td>MnCl2</td>
			<td>Merck</td>
			<td>429449</td>
			<td>Manganese(II) Chloride&#160;</td>
		</tr>
		<tr>
			<td>Na2 EDTA&#160;</td>
			<td>Merck</td>
			<td>200-449-4</td>
			<td>Edatamil, Edetato Disodium Salt Dihydrate</td>
		</tr>
		<tr>
			<td>Na2MoO4</td>
			<td>Merck</td>
			<td>243655</td>
			<td>Sodium Molybdate</td>
		</tr>
		<tr>
			<td>NaCl</td>
			<td>Meyer</td>
			<td>2365-500</td>
			<td>Sodium Chloride</td>
		</tr>
		<tr>
			<td>NaNO3</td>
			<td>Meyer</td>
			<td>2465-250</td>
			<td>Sodium Nitrate</td>
		</tr>
		<tr>
			<td>RGB LED stripe</td>
			<td>Steren</td>
			<td>GAD-LED2</td>
			<td>Light source</td>
		</tr>
		<tr>
			<td>Spectrophotometer</td>
			<td>PerkinElmer</td>
			<td>Model Lambda35</td>
			<td>Spectrophotometer</td>
		</tr>
		<tr>
			<td>spectroradiometer</td>
			<td>Gigahertz-Optik</td>
			<td>model BTS256</td>
			<td></td>
		</tr>
		<tr>
			<td>Vortex</td>
			<td>Scientific Industries</td>
			<td>Vortex-Genie&#174; 2</td>
			<td>Vortex</td>
		</tr>
		<tr>
			<td>ZnCl2</td>
			<td>Merck</td>
			<td>208086</td>
			<td>Zinc Chloride</td>
		</tr>
	</tbody>
</table>

evaluation of the effect of growth factors on chlorophylls a and b production from microalgae

In recent years, the growing environmental problems caused by anthropogenic activities and their adverse effects on the health and balance of ecosystems have driven the search for more efficient and environmentally friendly production systems. This has accelerated processes in industries and fostered the implementation of bioremediation treatments and the development of bio compounds to mitigate these harmful effects1.
This context has led to a significant growth in the study of microalgae, driven by the need to find innovative solutions to current environmental and economic challenges. Microalgae thrive in aquatic environments, using sunlight and carbon dioxide as sources of energy and carbon, respectively. This characteristic makes them a sustainable and promising alternative for producing various valuable products. Research in this field has focused on understanding the physiology and metabolism of these cells, as well as developing efficient technologies for their cultivation and processing2.
There is a clear need to develop accessible and reliable tools for studying microalgae to accelerate research processes and deepen the understanding of their physiology, metabolism, and potential applications. These tools should enable rapid analysis of the effect of environmental and production factors on key parameters, such as chlorophyll concentration, which is a fundamental indicator of the health and development of these organisms. A decrease in chlorophyll content may indicate environmental stress, nutritional deficiencies, or diseases.
These green pigments play a crucial role in photosynthesis, capturing sunlight energy to convert carbon dioxide and water into glucose and oxygen and constitute between 0.5% and 5% of the biomass of microalgae3. Beyond their essential role in sustaining life processes, chlorophylls have found applications in various industries. Chlorophyll extracts are utilized in food and beverage processing as natural colorants, imparting vibrant green hues to products while also providing antioxidant properties. Moreover, chlorophyll-based supplements are gaining popularity in the health and wellness sector due to their purported detoxifying and anti-inflammatory effects. By harnessing the multifaceted properties of chlorophyll, industries can develop innovative products that contribute to both visual appeal and consumer well-being1.
In this regard, one microalgae of great biotechnological relevance is C. sorokiniana. This microorganism stands out for its rapid growth rate, making it highly efficient in biomass production. Additionally, C. sorokiniana exhibits a diverse content of highly nutritional compounds, including proteins, lipids, and vitamins, which renders it valuable for various applications in food, feed, and biofuel production2. Moreover, this microalgae species has been found to produce extracellular enzymes with diverse functions, opening up possibilities for biotechnological applications such as wastewater treatment, bioremediation, and pharmaceuticals4. In addition to its rapid growth and versatile applications, C. sorokiniana also demonstrates significant potential for chlorophyll production. As a photosynthetic microorganism, C. sorokiniana possesses the machinery necessary for the synthesis of chlorophyll, which constitutes between 0.5% and 5% of the biomass of microalgae3. This capability makes C. sorokiniana an attractive candidate for commercial-scale chlorophyll production and promises to be a sustainable solution to urgent environmental and nutritional challenges5.
On the other hand, another microalgae species of significant interest is H. pluvialis. This microalga is renowned for its production of astaxanthin, a potent antioxidant pigment with numerous industrial applications. Astaxanthin serves as a protective mechanism for H. pluvialis photosystem, shielding it from oxidative stress induced by environmental factors. This pigment is highly sought after in cosmetics, nutraceuticals, and aquaculture industries, owing to its antioxidant properties and potential health benefits. With its abundant ability to produce astaxanthin, H. pluvialis presents a promising avenue for developing innovative products catering to various industrial and consumer needs6.
Recent studies have also highlighted H. pluvialis1 potential for chlorophyll production, further enhancing its industrial significance. For instance, a study investigated the chlorophyll content of H. pluvialis under varying growth conditions and found that under optimal cultivation parameters, H. pluvialis exhibited remarkable chlorophyll production rates, surpassing those of other microalgae species7. This finding underscores the potential of H. pluvialis as a sustainable source of chlorophyll, offering novel opportunities for its utilization in various industrial sectors.

<meta charset="utf8"></head><body>作者聚焦：优化生长因子以生产生物技术相关的次生代谢物

生长因子对微藻叶绿素 a 和 b 生产的影响评价

Our project evaluates how growth factor control affects the production of biotechnologically-relevant secondary metabolites such as chlorophyll from microalgae such as Chlorella sorokiniana and Haematococcus pluvialis at different scales. We have focused on establishing an analytical method to quantify chlorophylls in microalgae. Some emerging technologies for advancement in this are the modeling and optimization of processes using computational technique.However, in order to carry them out, it is necessary to obtain experimental information on the processes. The growth factors evaluated in Chlorella sorokiniana that have the greatest effect on chlorophyll production are the light color and the carbon dioxide supply flow. The changing light intensity does not significantly influence the production of this metabolite.The findings in this field of study can be advanced by implementing growth factors that induce microalgae to increase the production of secondary metabolites in conjunction with rapid, simple, and reliable analysis techniques. Our work provide those with guidelines to investigate and understand the biochemical and the energetic processes carried out by microalgae with different substrates, evaluating the effect of environmental variables to optimize the production of your mass and cellular compounds for various applications.

Microalgae contain two main groups of pigments: chlorophylls and carotenoids. Chlorophyll is a green pigment that absorbs light energy and transforms it ...

evaluation-effect-growth-factors-on-chlorophylls-b-production-from

Research

JoVE Journal

Biochemistry

Evaluation of the Effect of Growth Factors on Chlorophylls a and b Production from Microalgae

717 次观看.  National Institute of Technology of Mexico. 我们的项目评估了生长因子控制如何影响生物技术相关次生代谢物的产生，例如不同规模的微藻（如小球藻和雨生红球藻）的叶绿素。我们专注于建立一种分析方法来量化微藻中的叶绿素。在这方面取得进步的一些新兴技术是使用计算技术对过程进行建模和优化。但是，为了执行它们，有必要获取有关过程的实验信息。在 Chlorella sorokiniana 中评?...