Preparation and Fractionation of Xenopus laevis Egg Extracts
August 27th, 2008
•Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
Related Videos
Plastic Embedding and Sectioning of Xenopus laevis Embryos
Obtaining Eggs from Xenopus laevis Females
In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
Microinjection of Xenopus Laevis Oocytes
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
Facial Transplants in Xenopus laevis Embryos
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved