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Preparation and Fractionation of Xenopus laevis Egg Extracts

DOI :

10.3791/891-v

August 27th, 2008

August 27th, 2008

12,089 Views

1Department of Cell Biology, Emory University

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

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Preparation

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