Michael C. Jewett

Update on designing and building minimal cells.

Biology by design: from top to bottom and back.

Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.

Cell-free synthetic biology: thinking outside the cell.

Cell-free protein synthesis: Applications come of age.

Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry.

Synthetic in vitro circuits.

Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis.

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation.

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis.

Mapping Condition Dependent Regulation of Lipid Metabolism in Saccharomyces cerevisiae.

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis.

Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation.

A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe.

Quantifying position-dependent codon usage bias.

The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction.

Cell-free synthetic biology special issue.

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis.

Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis.

Characterizing and alleviating substrate limitations for improved in vitro ribosome construction.

Cell-free metabolic engineering: biomanufacturing beyond the cell.

Controlled assembly of artificial protein-protein complexes via DNA duplex formation.

High-throughput preparation methods of crude extract for robust cell-free protein synthesis.

Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1.

pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders.

Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction.

Energizing eukaryotic cell-free protein synthesis with glucose metabolism.

Self-Assembly of Temperature-Responsive Protein-Polymer Bioconjugates.

Repurposing the translation apparatus for synthetic biology.

Protein synthesis by ribosomes with tethered subunits.

Robust production of recombinant phosphoproteins using cell-free protein synthesis.

Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase.

A cell-free expression and purification process for rapid production of protein biologics.

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol.

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids.

Evolution of translation initiation sequences using in vitro yeast ribosome display.

Editorial: Transforming biotechnology with synthetic biology.

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery.

Synthetic biology: Tailor-made genetic codes.

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes.

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis.

In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis.

Depletion of Shine-Dalgarno Sequences Within Bacterial Coding Regions Is Expression Dependent.

Cell-Free Synthetic Biology: Engineering Beyond the Cell.

NullSeq: A Tool for Generating Random Coding Sequences with Desired Amino Acid and GC Contents.

Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.

Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency.

Establishing a high yielding streptomyces-based cell-free protein synthesis system.

Cogenerating Synthetic Parts toward a Self-Replicating System.

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies.

Repurposing ribosomes for synthetic biology.

A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases.

Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis.

Controlling cell-free metabolism through physiochemical perturbations.

Editorial overview: Synthetic biology: Frontiers in synthetic biology.

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases.

Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands.

Toward an orthogonal central dogma.

A novel framework for evaluating the performance of codon usage bias metrics.

Incorporation of Nonproteinogenic Amino Acids in Class I and II Lantibiotics.

How many human proteoforms are there?

A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology.

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids.

Engineered Ribosomes for Basic Science and Synthetic Biology.

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases.

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

BioBits™ Explorer: A modular synthetic biology education kit.

BioBits™ Bright: A fluorescent synthetic biology education kit.

Within-Gene Shine-Dalgarno Sequences Are Not Selected for Function.

Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

Cell-Free Synthetic Biology for Pathway Prototyping.

Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response.

Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry.