Frederick J. Ehlert

Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor.

Increased relaxant action of forskolin and isoproterenol against muscarinic agonist-induced contractions in smooth muscle from M2 receptor knockout mice.

Comparison of the pharmacological antagonism of M2 and M3 muscarinic receptors expressed in isolation and in combination.

Pharmacological analysis of the contractile role of M2 and M3 muscarinic receptors in smooth muscle.

Muscarinic agonist-mediated heterologous desensitization in isolated ileum requires activation of both muscarinic M2 and M3 receptors.

Contractile role of M2 and M3 muscarinic receptors in gastrointestinal, airway and urinary bladder smooth muscle.

Comparison of the antimuscarinic action of p-fluorohexahydrosiladifenidol in ileal and tracheal smooth muscle.

Functional analysis of muscarinic acetylcholine receptors using knockout mice.

The M2 muscarinic receptor mediates contraction through indirect mechanisms in mouse urinary bladder.

Analysis of allosterism in functional assays.

Determination of the rate of muscarinic M1 receptor plasma membrane delivery using a regulated secretion/aggregation system.

Differential coupling of muscarinic M1, M2, and M3 receptors to phosphoinositide hydrolysis in urinary bladder and longitudinal muscle of the ileum of the mouse.

Estimation of agonist activity at G protein-coupled receptors: analysis of M2 muscarinic receptor signaling through Gi/o,Gs, and G15.

Neuronally released acetylcholine acts on the M2 muscarinic receptor to oppose the relaxant effect of isoproterenol on cholinergic contractions in mouse urinary bladder.

Cysteine pairs in the third intracellular loop of the muscarinic m1 acetylcholine receptor play a role in agonist-induced internalization.

On the analysis of ligand-directed signaling at G protein-coupled receptors.

Two-state models and the analysis of the allosteric effect of gallamine at the M2 muscarinic receptor.

Use of acetylcholine mustard to study allosteric interactions at the M(2) muscarinic receptor.

Selectivity of agonists for the active state of M1 to M4 muscarinic receptor subtypes.

Estimation of relative microscopic affinity constants of agonists for the active state of the receptor in functional studies on M2 and M3 muscarinic receptors.

The guinea pig ileum lacks the direct, high-potency, M(2)-muscarinic, contractile mechanism characteristic of the mouse ileum.

A conserved motif in the membrane proximal C-terminal tail of human muscarinic m1 acetylcholine receptors affects plasma membrane expression.

Investigating the interaction of McN-A-343 with the M2 muscarinic receptor using its nitrogen mustard derivative.

Mutagenesis of nucleophilic residues near the orthosteric binding pocket of M1 and M2 muscarinic receptors: effect on the binding of nitrogen mustard analogs of acetylcholine and McN-A-343.

Analysis of agonism and inverse agonism in functional assays with constitutive activity: estimation of orthosteric ligand affinity constants for active and inactive receptor states.

Analysis of functional responses at G protein-coupled receptors: estimation of relative affinity constants for the inactive receptor state.

Characterization of muscarinic receptors in the human bladder mucosa: direct quantification of subtypes using 4-DAMP mustard.

Muscarinic agonists and antagonists: effects on gastrointestinal function.