standardized-histomorphometric-evaluation-osteoarthritis-surgical

Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model

One of the most prevalent joint disorders in the United States, osteoarthritis (OA) is characterized by progressive degeneration of articular cartilage, ...

Pennsylvania State College of Medicine

MicroRNAs (miRNAs) comprise a class of small non-coding RNAs that regulate the stability and/or translatability of most protein-coding transcripts. Steady-state levels of mature miRNAs can be controlled through mechanisms that influence their biogenesis and/or decay rates. Pathways that mediate mature miRNA decay are less well understood than those that mediate miRNA biogenesis. We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellular pathway that promotes the sequence-specific endonucleolytic decay of miRNAs that harbor a CA and/or UA dinucleotide. Here, we describe an in vitro assay for evaluating the susceptibility of AGO2-loaded miRNAs to degradation by different classes of nucleases. This in vitro approach can be used to complement in vivo studies that aim to identify novel miRNA decay factors.

Reyad Elbarbary

Center for Orthopedic Research and Translational Sciences,
Department of Orthopedics and Rehabilitation,
Department of Biochemistry and Molecular Biology,
Center for Orthopedic Research and Translational Sciences, Department of Orthopedics and Rehabilitation

Pennsylvania State College of Medicine

Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model