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Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

A biomimetic NM was developed to serve as a tissue-engineering biological scaffold, which can enhance stem cell anchorage. The biomimetic NM is formed ...

fabrication-characterization-layer-layer-janus-base-nano-matrix-to

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Various biomaterial scaffolds have been developed to guide cell adhesion and proliferation in hopes to promote specific functions for in vitro and in vivo ...

University of Connecticut

Mesenchymal stem cells (MSCs) have been widely applied in biomedicine due to their ability to differentiate into many different cell types and their ability to synthesize a broad spectrum of growth factors and cytokines that directly and indirectly influence other cells in their vicinity. To guide MSC infiltration to a bone fracture site, we developed a novel self-assembled Nano-Matrix which can be used as an injectable scaffold to repair bone fractures. The Nano-Matrix is formed by Janus base nanotubes (JBNTs) and fibronectin (FN). JBNTs are nucleobase-derived nanotubes mimicking collagen fibers, and FN is one of the cell adhesive glycoproteins which is responsible for cell-extracellular matrix interactions and guides stem cell migration and differentiation to desired cells types. Here, we demonstrated the successful fabrication and characterization of the JBNT/FN Nano-Matrix as well as its excellent bioactivity that encouraged human MSC migration and adhesion. This work lays a solid foundation for using the Nano-Matrix as an injectable approach to improve MSC retention and function during bone fracture healing.

Self-assembled biomimetic Nano-Matrix for stem cell anchorage.

Nanotechnology platforms, such as nanoparticles, liposomes, dendrimers, and micelles have been studied extensively for various drug deliveries, to treat or prevent diseases by modulating physiological or pathological processes. The delivery drug molecules range from traditional small molecules to recently developed biologics, such as proteins, peptides, and nucleic acids. Among them, proteins have shown a series of advantages and potential in various therapeutic applications, such as introducing therapeutic proteins due to genetic defects, or used as nanocarriers for anticancer agents to decelerate tumor growth or control metastasis. This review discusses the existing nanoparticle delivery systems, introducing design strategies, advantages of using each system, and possible limitations. Moreover, we will examine the intracellular delivery of different protein therapeutics, such as antibodies, antigens, and gene editing proteins into the host cells to achieve anticancer effects and cancer vaccines. Finally, we explore the current applications of protein delivery in anticancer treatments.

Nanomaterials for Protein Delivery in Anticancer Applications.

Degenerative Disc Disease (DDD) causes a nagging to severe back pain as well as numbing sensation to the extremities leading to loss of overall patients' height and weakness to leg muscles. Degenerative disc disease is often observed in aging patients as well as patients who have suffered from a back injury. Cervical Degenerative Disc Disease (CDDD) is a progressive condition that leads to the degeneration of the intervertebral discs supporting the cervical vertebral column. Anterior Cervical Interbody Fusion (ACIF) has been the longstanding treatment option for severe degenerative disc disease; however, ACIF presents various novel complications, necessitating numerous comparative device studies to reduce the negative effects of spinal fusion. Cervical disc arthroplasty, the recent focus of clinical attention, was one of the alternatives studied to mitigate the complications associated with vertebral fusion but presents its own disadvantages. These complications prompted further investigation and modifications that can be introduced into these devices. We will be discussing the nano-scale interactions between the implant and extracellular matrix play a crucial role in device integration and efficacy, providing an additional approach towards a device's overall success.

Anne Yau

Department of Biomedical Engineering

University of Connecticut

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Anne Yau

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University of Connecticut

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Department of Biomedical Engineering