quantification-cellular-densities-antigenic-properties-using-magnetic

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

The described method was developed based on the principles of magnetic levitation, which separates cells and particles based on their density and magnetic ...

Beth Israel Deaconess Medical Center

Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field. We describe a novel magnetic levitation-based method that can specifically detect cell membrane-bound and soluble antigens by measurable changes in levitation height that result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein-Barr viral particles, and soluble IL-6, and validate the results by flow cytometry and immunofluorescence microscopy performed in parallel. Additionally, employing an inexpensive, single lens, manual focus, wifi-enabled camera, we extend the portability of our method for its potential use as a point-of-care diagnostic assay.

Mark Gregory

Department of Medicine,
Division of Allergy and Inflammation,
Department of Medicine, Division of Allergy and Inflammation

Beth Israel Deaconess Medical Center

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

Mark Gregory

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Medicine,Division of Allergy and Inflammation,Department of Medicine, Division of Allergy and Inflammation

Beth Israel Deaconess Medical Center

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

Department of Medicine,
Division of Allergy and Inflammation,
Department of Medicine, Division of Allergy and Inflammation