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Cincinnati Children’s Hospital Medical Center, Cincinnati

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

Rory Turner^*1, Rajib Mukherjee^*2, Martina Wallace¹, Joan Sanchez-Gurmaches^2,3,4

¹UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, ²Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, ³Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, ⁴Department of Pediatrics, University of Cincinnati College of Medicine

Here, we present an inexpensive quantitative method utilizing deuterium oxide and gas chromatography mass spectrometry (GCMS) for the analysis of total fatty acid de novo lipogenesis in brown adipose tissue in vivo.