Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear CellsFabian Dieter 1, Jendrik Grube 1, Tim Birkenhauer 1, Alice Quentin 1, Gunter P. Eckert 1
1Biomedical Research Center, Institute of Nutritional Sciences, Justus-Liebig-University of Giessen
Isolated peripheral blood mononuclear cells can be used for the analysis of immune functions and disorders, metabolic diseases, or mitochondrial functions. In this work, we describe a standardized method for the preparation of PBMCs from whole blood and the subsequent cryopreservation. Cryopreservation makes this time and place independent.
