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Justus-Liebig-University of Giessen

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Fabian Dieter¹, Jendrik Grube¹, Tim Birkenhauer¹, Alice Quentin¹, Gunter P. Eckert¹

¹Biomedical Research Center, Institute of Nutritional Sciences, Justus-Liebig-University of Giessen

Isolated peripheral blood mononuclear cells can be used for the analysis of immune functions and disorders, metabolic diseases, or mitochondrial functions. In this work, we describe a standardized method for the preparation of PBMCs from whole blood and the subsequent cryopreservation. Cryopreservation makes this time and place independent.