DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep LearningChangyue Liu *1,2, Zhengyang Lei *1,2, Lijin Lian 2, Likun Zhang 1,2, Zhicheng Du 1,2, Peiwu Qin 1,2
1Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, 2Institute of Biopharmaceutics and Health Engineering, Tsinghua Shenzhen International Graduate School
We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.
