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Function Therapeutics

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader

Jacob T. DeRousse^1,2,3, Chris Dockendorff^1,2

¹Function Therapeutics, ²Department of Chemistry, Marquette University, ³School of Medicine, Washington University

An improved protocol for a calcium mobilization assay with endothelial cells, used to identify ligands of protease-activated receptors (PARs), has been developed. The new protocol reduces total assay time by 90-120 min and yields reproducible concentration-response curves.