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Kobe University

3 ARTICLES PUBLISHED IN JoVE

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Environment

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
Masaki Miya 1, Toshifumi Minamoto 2, Hiroki Yamanaka 3, Shin-ichiro Oka 4, Keiichi Sato 4, Satoshi Yamamoto 2, Tetsuya Sado 1, Hideyuki Doi 5
1Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment, Kobe University, 3Faculty of Science and Technology, Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies, University of Hyogo

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

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Biology

Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
Takehiro Kazama 1,2,3, Kazuhide Hayakawa 4, Koichi Shimotori 1,2, Akio Imai 1
1Lake Biwa Branch Office, National Institute for Environmental Studies, 2Center for Regional Environmental Research, National Institute for Environmental Studies, 3Graduate School of Human Development and Environment, Kobe University, 4Lake Biwa Environmental Research Institute

Fast repetition rate fluorometer (FRRf) is a beneficial method for measuring photosystem II photophysiology and primary productivity. Here we describe a protocol to measure PSII photophysiology of epizoic alga, Colacium sp. on substrate zooplankton using cuvette-type FRRf.

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Neuroscience

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
Daisuke Kato 1,2, Xiangyu Quan 3, Yuta Tanisumi 1,2, Zhongtian Guo 1,2, Mitsuhiro Morita 4, Tetsuya Takiguchi 5, Osamu Matoba 6, Hiroaki Wake 1,2,6
1Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 3Department of System Science, Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences, Kobe University, 5Department of Information Science, Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science, Kobe University

We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.

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