Kobe University

3 ARTICLES PUBLISHED IN JoVE

Environment

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Masaki Miya¹, Toshifumi Minamoto², Hiroki Yamanaka³, Shin-ichiro Oka⁴, Keiichi Sato⁴, Satoshi Yamamoto², Tetsuya Sado¹, Hideyuki Doi⁵

¹Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, ²Graduate School of Human Development and Environment, Kobe University, ³Faculty of Science and Technology, Ryukoku University, ⁴Okinawa Churashima Research Center, ⁵Graduate School of Simulation Studies, University of Hyogo

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

Biology

Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer

Takehiro Kazama^1,2,3, Kazuhide Hayakawa⁴, Koichi Shimotori^1,2, Akio Imai¹

¹Lake Biwa Branch Office, National Institute for Environmental Studies, ²Center for Regional Environmental Research, National Institute for Environmental Studies, ³Graduate School of Human Development and Environment, Kobe University, ⁴Lake Biwa Environmental Research Institute

Fast repetition rate fluorometer (FRRf) is a beneficial method for measuring photosystem II photophysiology and primary productivity. Here we describe a protocol to measure PSII photophysiology of epizoic alga, Colacium sp. on substrate zooplankton using cuvette-type FRRf.

Neuroscience

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Daisuke Kato^1,2, Xiangyu Quan³, Yuta Tanisumi^1,2, Zhongtian Guo^1,2, Mitsuhiro Morita⁴, Tetsuya Takiguchi⁵, Osamu Matoba⁶, Hiroaki Wake^1,2,6

¹Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, ²Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, ³Department of System Science, Kobe University Graduate School of System Informatics, ⁴Department of Biology, Graduate School of Sciences, Kobe University, ⁵Department of Information Science, Kobe University Graduate School of System Informatics, ⁶Center of Optical Scattering Image Science, Kobe University

We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.