MDI Biological Laboratory
2 ARTICLES PUBLISHED IN JoVE
Biology
Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
Georgeann S. O'Brien 1, Sandra Rieger 1, Seanna M. Martin 1, Ann M. Cavanaugh 1, Carlos Portera-Cailliau 2, Alvaro Sagasti 1
1Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, 2Departments of Neurology and Neurobiology, University of California, Los Angeles
Here we describe a method for mounting zebrafish embryos for long-term imaging, two-photon imaging and tissue-damage techniques, and time-lapse confocal imaging.
Developmental Biology
Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
Thomas S. Lisse 1, Elizabeth A. Brochu 1, Sandra Rieger 1
1Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory
We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
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