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MDI Biological Laboratory

2 ARTICLES PUBLISHED IN JoVE

Biology

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Georgeann S. O'Brien¹, Sandra Rieger¹, Seanna M. Martin¹, Ann M. Cavanaugh¹, Carlos Portera-Cailliau², Alvaro Sagasti¹

¹Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, ²Departments of Neurology and Neurobiology, University of California, Los Angeles

Here we describe a method for mounting zebrafish embryos for long-term imaging, two-photon imaging and tissue-damage techniques, and time-lapse confocal imaging.

Developmental Biology

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Thomas S. Lisse¹, Elizabeth A. Brochu¹, Sandra Rieger¹

¹Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory

We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.