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University Hospital Muenster

2 ARTICLES PUBLISHED IN JoVE

Medicine

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

Wen-Jia Liu^*1,2, Lisa Ernst^*2, Benedict Doorschodt², Jan Bednarsch¹, Felix Becker³, Richi Nakatake², Yuki Masano², Ulf Peter Neumann^1,4, Sven Arke Lang¹, Peter Boor⁵, Isabella Lurje⁶, Georg Lurje^1,6, René Tolba², Zoltan Czigany^1,2

¹Department of Surgery and Transplantation, Faculty of Medicine, University Hospital RWTH Aachen, ²Institute for Laboratory Animal Science, Faculty of Medicine, University Hospital RWTH Aachen, ³Department of General-, Visceral-, and Transplantation Surgery, University Hospital Muenster, ⁴Department of Surgery, Maastricht University Medical Centre (MUMC), ⁵Institute of Pathology, Faculty of Medicine, University Hospital RWTH Aachen, ⁶Department of Surgery, Campus Charité Mitte | Campus Virchow-Klinikum, Charité-Universitätsmedizin

Large animal models play an essential role in preclinical transplantation research. Due to its similarities to the clinical setup, the porcine model of orthotopic kidney auto-transplantation described in this article provides an excellent in vivo setting for the testing of organ preservation techniques and therapeutic interventions.

Neuroscience

Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice

Christina B. Schroeter^*1, Antonia Henes^*1, Anna Vogelsang¹, Alexander M. Herrmann¹, Stefanie Lichtenberg¹, Derya Cengiz¹, Vera Dobelmann¹, Niklas Huntemann¹, Christopher Nelke¹, Susann Eichler², Philipp Albrecht¹, Sven G. Meuth^*1, Tobias Ruck¹

¹Department of Neurology, Medical Faculty, Heinrich Heine University Duesseldorf, ²Department of Neurology, Institute of Translational Neurology, University Hospital Muenster

To date, protocols for the simultaneous isolation of all principal central nervous system-resident cell types from the same mouse are an unmet demand. The protocol shows a procedure applicable in naïve and experimental autoimmune encephalomyelitis mice to investigate complex cellular networks during neuroinflammation and simultaneously reduce the required mice numbers.