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Instituto Tecnologia Química e Biológica UNL, Av. da República

1 ARTICLES PUBLISHED IN JoVE

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Biology

Processing the Loblolly Pine PtGen2 cDNA Microarray
W. Walter Lorenz 1, Yuan-Sheng Yu 1, Marta Simões 2, Jeffrey F. D. Dean 1
1Warnell School of Forestry and Natural Resources, University of Georgia (UGA), 2Instituto de Biologia Experimental e Tecnológica, Instituto Tecnologia Química e Biológica UNL, Av. da República

The cDNA microarray PtGen2 was developed for gene expression studies in loblolly pine, P. taeda, and other conifer species. Here, we show pre- and post-hybridization handling and washing techniques that can be used with this array to yield better consistency, reduced artifacts, and lower backgrounds.

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