Bioenergetic Profile Experiment using C2C12 Myoblast CellsDavid G. Nicholls 1, Victor M. Darley-Usmar 2, Min Wu 3, Per Bo Jensen 3, George W. Rogers 3, David A. Ferrick 3
1Buck Institute for Age Research, Novato, CA, 2Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham - UAB, 3Seahorse Bioscience, North Billerica, MA
A description of a method for profiling mitochondrial function in cells is provided. The mitochondrial profile generated provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity.
