Tunghai University
2 ARTICLES PUBLISHED IN JoVE
Biology
Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
Yuan-Hao Hsu 1, Jolinda A. Traugh 2
1Department of Chemistry, Tunghai University, 2Department of Biochemistry, University of California, Riverside
MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.
Developmental Biology
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
Chih-Wei Chou 1, Jamie Lin 1, Hsin-Yu Hou 1, Yi-Wen Liu 1
1Department of Life Science, Tunghai University
The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.
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