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Tunghai University

2 ARTICLES PUBLISHED IN JoVE

Biology

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

¹Department of Chemistry, Tunghai University, ²Department of Biochemistry, University of California, Riverside

MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.

Developmental Biology

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Chih-Wei Chou¹, Jamie Lin¹, Hsin-Yu Hou¹, Yi-Wen Liu¹

¹Department of Life Science, Tunghai University

The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ^5-4isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.