Anmelden

Renovo Neural Incorporated

2 ARTICLES PUBLISHED IN JoVE

Biology

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Diego Bohórquez¹, Fariha Haque², Satish Medicetty³, Rodger A. Liddle¹

¹Department of Medicine, Duke University Medical Center, ²Department of Chemistry, University of North Carolina - Chapel Hill, ³Renovo Neural Incorporated

Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.

Neuroscience

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Konark Mukherjee¹, Helen R. Clark¹, Vrushali Chavan¹, Emily K. Benson², Grahame J. Kidd², Sarika Srivastava¹

¹Virginia Tech Carilion Research Institute, ²Renovo Neural Incorporated

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.