Renovo Neural Incorporated
2 ARTICLES PUBLISHED IN JoVE
Biology
Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
Diego Bohórquez 1, Fariha Haque 2, Satish Medicetty 3, Rodger A. Liddle 1
1Department of Medicine, Duke University Medical Center, 2Department of Chemistry, University of North Carolina - Chapel Hill, 3Renovo Neural Incorporated
Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy.
Neuroscience
Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
Konark Mukherjee 1, Helen R. Clark 1, Vrushali Chavan 1, Emily K. Benson 2, Grahame J. Kidd 2, Sarika Srivastava 1
1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated
Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.
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