An Easy Method for Plant Polysome ProfilingCécile Lecampion 1,2,3, Maïna Floris 1,2,3,4, Jean Raphaël Fantino 5,6, Christophe Robaglia 1,2,3, Christophe Laloi 1,2,3
1Laboratoire de Génétique et Biophysique des Plantes, Aix-Marseille Université, 2UMR 7265 Biologie Végétale & Microbiologie Environnementales, CNRS, 3BIAM, CEA, 4Department of Biology, Biocenter, University of Copenhagen, 5Laboratoire de Chimie Bactérienne, 6CNRS, LCB UMR 7283, Aix Marseille Université
This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.
