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Friedrich Schiller University

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Investigations on the Ga(III) Complex of EOB-DTPA and Its ⁶⁸Ga Radiolabeled Analogue

Julia Greiser¹, Tobias Niksch¹, Wolfgang Weigand², Martin Freesmeyer¹

¹Clinic of Nuclear Medicine, University Hospital Jena, ²Friedrich Schiller University

A procedure for the isolation of EOB-DTPA and subsequent complexation with natural Ga(III) and ⁶⁸Ga is presented herein, as well as a thorough analysis of all compounds and investigations on labeling efficiency, in vitro stability and the n-octanol/water distribution coefficient of the radiolabeled complex.

Immunology and Infection

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

Adrian Feile^*1,2,3, Valentin D. Wegner^*1, Martin Raasch⁴, Alexander S. Mosig^1,2,3,5

¹Institute of Biochemistry II, Jena University Hospital, ²Cluster of Excellence Balance of the Microverse, Friedrich Schiller University, ³Jena School of Microbial Communication, Friedrich Schiller University, ⁴Dynamic42 GmbH, ⁵Jena Center for Sepsis Control and Care, Jena University Hospital

Our detailed protocol outlines the creation and use of the advanced intestine-on-chip model, which simulates human intestinal mucosa with 3D structures and various cell types, enabling in-depth analysis of immune responses and cellular functions in response to microbial colonization.