Anmelden

RIKEN Cluster for Pioneering Research

1 ARTICLES PUBLISHED IN JoVE

JoVE Core

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Mari Mito^1,2, Mitsutaka Kadota³, Shinichi Nakagawa^1,4, Shintaro Iwasaki^2,5

¹RNA Biology Laboratory, RIKEN, ²RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, ³Laboratory for Phyloinformatics, RIKEN Center for Biosystems Dynamics Research, ⁴RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, ⁵Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo

We describe a step-by-step protocol for tandem chromatin immunoprecipitation sequencing (tChIP-Seq) that enables the analysis of cell-type-specific genome-wide histone modification.