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ProMab Biotechnologies. Inc

2 ARTICLES PUBLISHED IN JoVE

Medicine

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

Hui-Wen Cheng^*1,2, Sudeshna Fisch^*1, Susan Cheng¹, Michael Bauer¹, Soeun Ngoy¹, Yiling Qiu¹, Jian Guan¹, Shikha Mishra¹, Christopher Mbah¹, Ronglih Liao¹

¹Cardiac Muscle Research Labratory, Cardiovascular Division, Brigham and Women’s Hospital, Harvard Medical School, ²Cardiovascular Department, Chang Gung Memorial Hospital

Right ventricle (RV) dysfunction is critical to the pathogenesis of cardiovascular disease, yet limited methodologies are available for its evaluation. Recent advances in ultrasound imaging provide a noninvasive and accurate option for longitudinal RV study. Herein, we detail a step-by-step echocardiographic method using a murine model of RV pressure overload.

Immunology and Infection

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Biao Xi¹, Robert Berahovich², Hua Zhou², Shirley Xu², Yuehua Wei², Jasper Guan², Hizkia Harto², Jian Guan², Lijun Wu², David Santa Ana^1,3, Fabio Cerignoil¹, Brandon Lamarche¹, Yama A. Abassi¹, Vita Golubovskaya²

¹ACEA Biosciences, Inc, ²ProMab Biotechnologies. Inc, ³ACEA Therapeutics

We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.