ProMab Biotechnologies. Inc

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography
Hui-Wen Cheng *1,2, Sudeshna Fisch *1, Susan Cheng 1, Michael Bauer 1, Soeun Ngoy 1, Yiling Qiu 1, Jian Guan 1, Shikha Mishra 1, Christopher Mbah 1, Ronglih Liao 1
1Cardiac Muscle Research Labratory, Cardiovascular Division, Brigham and Women’s Hospital, Harvard Medical School, 2Cardiovascular Department, Chang Gung Memorial Hospital

Right ventricle (RV) dysfunction is critical to the pathogenesis of cardiovascular disease, yet limited methodologies are available for its evaluation. Recent advances in ultrasound imaging provide a noninvasive and accurate option for longitudinal RV study. Herein, we detail a step-by-step echocardiographic method using a murine model of RV pressure overload.

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Immunology and Infection

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
Biao Xi 1, Robert Berahovich 2, Hua Zhou 2, Shirley Xu 2, Yuehua Wei 2, Jasper Guan 2, Hizkia Harto 2, Jian Guan 2, Lijun Wu 2, David Santa Ana 1,3, Fabio Cerignoil 1, Brandon Lamarche 1, Yama A. Abassi 1, Vita Golubovskaya 2
1ACEA Biosciences, Inc, 2ProMab Biotechnologies. Inc, 3ACEA Therapeutics

We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.

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