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2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
Julia Peics *1, Lavanya Vishvanath *2, Qianbin Zhang 2, Bo Shan 2, Thomas Å. Pedersen 1, Rana K. Gupta 2
1Diabetes Pharmacology, Novo Nordisk A/S, 2Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center

This protocol describes the technical approach to isolate adipogenic and fibro-inflammatory stromal cell subpopulations from murine intra-abdominal white adipose tissue (WAT) depots by fluorescence-activated cell sorting or immunomagnetic bead separation.

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Biology

Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice
Ariadna Carol Illa 1,2, Sarah Baumgarten 1, Dennis Danielsen 1, Karin Larsen 3, Torben Elm 1, Peter B. Johansen 4, Tom Knudsen 5, Brian Lauritzen 1, Mikael Tranholm 6, Carsten D. Ley 1
1Global Drug Discovery, Novo Nordisk A/S, 2Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 3Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 4Independent consultant, 5Catalyst Biosciences, 6Værløse Dyreklinik

The refined tail vein transection (TVT) bleeding model in anesthetized mice is a sensitive in vivo method for the assessment of hemophilic bleeding. This optimized TVT bleeding model uses blood loss and bleeding time as endpoints, refining other models and avoiding death as an endpoint.

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