Novo Nordisk A/S

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Julia Peics^*1, Lavanya Vishvanath^*2, Qianbin Zhang², Bo Shan², Thomas Å. Pedersen¹, Rana K. Gupta²

¹Diabetes Pharmacology, Novo Nordisk A/S, ²Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center

This protocol describes the technical approach to isolate adipogenic and fibro-inflammatory stromal cell subpopulations from murine intra-abdominal white adipose tissue (WAT) depots by fluorescence-activated cell sorting or immunomagnetic bead separation.

Biology

Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice

Ariadna Carol Illa^1,2, Sarah Baumgarten¹, Dennis Danielsen¹, Karin Larsen³, Torben Elm¹, Peter B. Johansen⁴, Tom Knudsen⁵, Brian Lauritzen¹, Mikael Tranholm⁶, Carsten D. Ley¹

¹Global Drug Discovery, Novo Nordisk A/S, ²Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, ³Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, ⁴Independent consultant, ⁵Catalyst Biosciences, ⁶Værløse Dyreklinik

The refined tail vein transection (TVT) bleeding model in anesthetized mice is a sensitive in vivo method for the assessment of hemophilic bleeding. This optimized TVT bleeding model uses blood loss and bleeding time as endpoints, refining other models and avoiding death as an endpoint.