<h2 class="jove_title"><a href="https://www.jove.com/video/4326">Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse</a></h2>
Holly A. Robinson, SunKuk Kwon, Mary A. Hall, John C. Rasmussen, Melissa B. Aldrich, Eva M. Sevick-Muraca Center for Molecular Imaging (CMI), University of Texas Health Science Center-Houston
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
<h2 class="jove_title"><a href="https://www.jove.com/video/4379">Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants</a></h2>
Pei-Yi Lin1, Nadege Roche-Labarbe1, 2, Mathieu Dehaes3, Stefan Carp1, Angela Fenoglio3, Beniamino Barbieri4, Katherine Hagan1, P. Ellen Grant3, Maria Angela Franceschini1 1Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 2Lab. PALM, Universit&#233; de Caen Basse-Normandie, 3Fetal-Neonatal Neuroimaging and Developmental Science Center, Boston Children's Hospital, Harvard Medical School, 4ISS, INC.
We combined frequency-domain near-infrared spectroscopy measures of cerebral hemoglobin oxygenation with diffuse correlation spectroscopy measures of cerebral blood flow index to estimate an index of oxygen metabolism. We tested the utility of this measure as a bedside screening tool to evaluate the health and development of the newborn brain.
<h2 class="jove_title"><a href="https://www.jove.com/video/50096">Reduction In Left Ventricular Wall Stress And Improvement In Function In Failing Hearts Using Algisyl-LVR</a></h2>
Lik Chuan Lee1, Zhang Zhihong1, Andrew Hinson2, Julius M. Guccione1 1Department of Surgery, UCSF/VA Medical Center, 2Clinical &amp; Regulatory, LoneStar Heart, Inc.
This article describes procedures for implanting a novel hydrogel in failing hearts and quantifying its effect on left ventricular wall stress and function. These procedures have been successfully applied in dogs and humans.
<h2 class="jove_title"><a href="https://www.jove.com/video/50272">Development Of An Audio-based Virtual Gaming Environment To Assist With Navigation Skills In The Blind</a></h2>
Erin C. Connors1, Lindsay A. Yazzolino1, Jaime S&#225;nchez2, Lotfi B. Merabet1 1Laboratory for Visual Neuroplasticity, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 2Department of Computer Science and Center for Advanced Research in Education (CARE), University of Chile 

<h2 class="jove_title"><a href="https://www.jove.com/video/4325">Determining 3D Flow Fields Via Multi-camera Light Field Imaging</a></h2>
Tadd T. Truscott1, Jesse Belden2, Joseph R. Nielson1, David J. Daily1, Scott L. Thomson1 1Department of Mechanical Engineering, Brigham Young University, 2Naval Undersea Warfare Center, Newport, RI
A technique for performing quantitative three-dimensional (3D) imaging for a range of fluid flows is presented. Using concepts from the area of Light Field Imaging, we reconstruct 3D volumes from arrays of images. Our 3D results span a broad range including velocity fields and multi-phase bubble size distributions.

march 2013: this month in jove

Hier sind einige Highlights aus dem März 2013 Ausgabe des Journal of Visualized Experiments (Jupiter). In diesem Monat JoVE verfügt über mehrere Video-Artikel in der Clinical and Translational Medicine Abschnitt. In der Augenabteilung, Connors et al. of Massachusetts Eye &amp; Ear und der Harvard Medical School haben eine Audio-basierten virtuellen Umgebung Simulator zu helfen blinden Menschen verbessern ihre Navigation Fähigkeiten entwickelt. Der Simulator ermöglicht es Benutzern, kognitiven Karten von dreidimensionalen Zwischenräumen im Kontext eines Videospiels unter Verwendung nur-Audiohinweisen bauen. Die Nutzer nicht bewusst waren, dass die virtuelle Gebäude im Spiel eine tatsächliche Gebäude stellt, außerdem die Anwender wurden nie gefragt, um das Gebäude Layout erinnern während des Spielens. Dennoch scheint es, dass das Spiel kann ihnen helfen, durch unbekannte Räume zu navigieren. Am Massachusetts General Hospital und der Harvard Medical School, Lin et al. entwickeln eine verbesserte meThSB zur nichtinvasiven Messung Stoffwechsel im Gehirn und des Blutflusses bei Neugeborenen. Dieses Verfahren kombiniert Messungen der Hämoglobin-Sauerstoffsättigung (SO 2) mit Hirndurchblutung Index (CBF i), die genaue Messungen der zerebralen Stoffwechsel und hämodynamischen bereitstellt. Diese Methode entspricht auch neonatale ICU Politik, so dass es möglicherweise nützlich für die Beurteilung der Gesundheit des Gehirns, Entwicklung und Ansprechen auf die Therapie bei Neugeborenen. Auch in diesem Abschnitt, Lee et al. der UC San Francisco / VA Medical Center zeigen, wie ein Biopolymer-Gel, aus Polysacchariden in Braunalgen gefunden abgeleitet, können Patienten mit Herzinsuffizienz helfen. Das Alginat-basierten Biopolymer ist in dem Bereich zwischen der Basis und der Spitze der linken Herzkammer freien Wand eingespritzt. Dies reduziert linken Ventrikels Größe und verdickt sich ihre freien Wand, sondern reduziert auch die linksventrikuläre Wandspannung, wie durch mathematische Modellierung beurteilt. Diese Therapie, genannt Algisyl-LVR, befindet sich derzeit in der klinischen Entwicklungfür die Behandlung von Patienten mit dilatativer Kardiomyopathie. In JoVE Immunologie und Infektionsbiologie, Robinson et al. der University of Texas Health Science Center-Houston zeigen eine nicht-invasive Methode zur Bildgebung Lymphgefäße, die in vielen physiologischen und Krankheit beteiligt sind. Im nahen Infrarot fluoreszierende Farbstoff wie Indocyaningrün (ICG), intradermal injiziert wird-so dass das lymphatische Gefäßsystem zu visualisierenden Verwendung eines Nah-Infrarot-Fluoreszenz-Imaging-System. Die JoVE Applied Physics Sektion verfügt über eine Methode auf dem Gebiet der Strömungsmechanik, an der Brigham Young University in Zusammenarbeit mit dem Naval Undersea Warfare Center in Newport, Rhode Island entwickelt. Truscott et al. beschreiben eine neuartige Technik zur quantitativen dreidimensionalen Bildgebung des Fluidflusses Feldern wie den hindurchtretenden Luftstrom über einen Satz von synthetischen Stimmlippen oder Blasenströmung Felder. Diese Methode kann helfen, eine Reihe von Problemen im Bereichder Strömungsmechanik. Diese Zusammenfassung bietet eine Vorschau auf die hochmoderne Video-Publikationen erscheinen in diesem Monat in JoVE. Besuchen Sie die Website und überprüfen Sie die Artikel in voller Länge, plus viele weitere, in JoVE: The Journal of Visualized Experiments.

Watch this Scientific Journal Video about March 2013: This Month in JoVE at JoVE.com

March 2013: This Month in JoVE

Hier sind einige Highlights aus dem März 2013 Ausgabe des Journal of Visualized Experiments (Jupiter).

März 2013: Diesen Monat in JoVE

Here are some highlights from the March 2013 issue of Journal of Visualized Experiments (JoVE).
This month, JoVE features several video articles in the ...

Research

JoVE Journal

Biology

9.0K Aufrufe.  Massachusetts Eye and Ear. Hier sind einige Highlights aus dem März 2013 Ausgabe des Journal of Visualized Experiments (Jupiter).

Serie: March 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

August 2012: Diesen Monat in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Forschung

Biologie

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Das Jahr im Rückblick

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...