<h2 class="jove_title"><a href="https://www.jove.com/video/4367">The Resident-intruder Paradigm: A Standardized Test for Aggression, Violence and Social Stress</a></h2>
Jaap M. Koolhaas1, Caroline M. Coppens1, Sietse F. de Boer1, Bauke Buwalda1, Peter Meerlo1, Paul J.A. Timmermans2
1Department of Behavioral Physiology, Center for Behavior and Neurosciences, University Groningen, 2Radboud University Nijmegen
This video shows the resident-intruder paradigm in rats. This test is a standardized method to measure offensive aggression, defensive behavior and violence in a semi natural setting. The use of the paradigm for social stress experiments is explained as well.
<h2 class="jove_title"><a href="https://www.jove.com/video/50268">Designing a Bio-responsive Robot from DNA Origami</a></h2>
Eldad Ben-Ishay, Almogit Abu-Horowitz, Ido Bachelet
<a href='/institutions/AS-asia/IL-israel/574-bar-ilan-university'>Faculty of Life Sciences and the Institute for Nanotechnology &#38; Advanced Materials, Bar-Ilan University</a>
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here we describe how DNA origami can be utilized to design a robotic robot capable of sensing biological cues and responding by shape shifting, subsequently relayed to a desired effect.
<h2 class="jove_title"><a href="https://www.jove.com/video/50509">The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis</a></h2>
Rishu Gupta1,2, Maya Debbaneh2,3, Daniel Butler2,4, Monica Huynh2,5, Ethan Levin2, Argentina Leon2, John Koo2, Wilson Liao2
1<a href='/institutions/NA-north-america/US-united-states/CA-california/16311-university-of-southern-california'>Keck School of Medicine, University of Southern California</a>, 2<a href='/institutions/NA-north-america/US-united-states/CA-california/23335-university-of-california-san-francisco'>Psoriasis and Skin Treatment Center Dermatology, University of California, San Francisco</a>, 3University of California Irvine School of Medicine, 4University of Arizona College of Medicine, 5Chicago College of Osteopathic Medicine
Psoriasis is a chronic, immune-mediated inflammatory skin disease. The Goeckerman regimen, formulated for the treatment of psoriasis, consists of exposure to ultraviolet B (UVB) light and application of crude coal tar (CCT). The following protocol is for the administration of Goeckerman therapy for the treatment of moderate-to-severe psoriasis.
<h2 class="jove_title"><a href="https://www.jove.com/video/50521">Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins</a></h2>
Kahlin Leuzinger*, Matthew Dent*, Jonathan Hurtado, Jake Stahnke, Huafang Lai, Xiaohong Zhou, Qiang Chen
<a href='/institutions/NA-north-america/US-united-states/AZ-arizona/19026-arizona-state-university'>The College of Technology and Innovation, Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University</a>
Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.
<h2 class="jove_title"><a href="https://www.jove.com/video/50539">Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae</a></h2>
Darc&#233;e D. Sloboda1, Dennis R. Claflin1,2, James J. Dowling3, Susan V. Brooks1,4
1<a href='/institutions/NA-north-america/US-united-states/MI-michigan/1888-university-of-michigan-ann-arbor'>Department of Biomedical Engineering, University of Michigan</a>, 2<a href='/institutions/NA-north-america/US-united-states/MI-michigan/1888-university-of-michigan-ann-arbor'>Department of Surgery, Section of Plastic Surgery, University of Michigan</a>, 3<a href='/institutions/NA-north-america/US-united-states/MI-michigan/1888-university-of-michigan-ann-arbor'>Departments of Pediatrics and Neurology, University of Michigan</a>, 4<a href='/institutions/NA-north-america/US-united-states/MI-michigan/1888-university-of-michigan-ann-arbor'>Department of Molecular and Integrative Physiology, University of Michigan</a>
Force measurements can be used to demonstrate changes in muscle function due to development, injury, disease, treatment or chemical toxicity. In this video, we demonstrate a method to measure force during a maximal contraction of zebrafish larval trunk muscle.

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july 2013: this month in jove

Hier ist ein Blick auf, was kommt im <a href="http://www.jove.com/archive/77" target="_blank">Juli 2013 Ausgabe</a> von <a href="http://www.jove.com/" target="_blank">JoVE: The Journal of Visualized Experiments.</a> In diesem Monat nehmen wir ein Bad im Zebrafisch Tank und verwenden Sie dieses klassische Labor Modell Muskelfunktion zu studieren. <a href="http://www.jove.com/video/50539" target="_blank">Sloboda, et al.</a> Maßnahme Krafterzeugung während Stamm Muskelkontraktion in Zebrafisch-Larven. Dies wird durch das Anbringen der Larven zu einem Kraftaufnehmer und einer Länge Controller getan; die Autoren dann gelten ein elektrisches Feld an die Muskelfasern zu stimulieren, und bestimmen Sie die Kraft erzeugenden Kapazität der Larven Muskeln. Daraus ergibt sich eine sinnvolle Maßnahme der Muskelfunktion und die allgemeine Gesundheit. <a href="http://www.jove.com/video/50521" target="_blank">Leuzinger, et al.</a> kombinieren Landwirtschaft mit pharma in Demonstrating einer transgenen pflanzlichen System zur Expression von Biopharmazeutika in einem landwirtschaftlichen Maßstab. Die Autoren agroinfiltrate Pflanzenblätter mit viralen Vektoren auf hohem Niveau transiente Expression von rekombinanten Proteinen, die zu großen pharmazeutischen Produktion führen zu weltweit steigenden Anforderungen gerecht zu induzieren. Bewegen Sie sich auf <a href="http://www.jove.com/behavior?utm_source=tmij" target="_blank">JoVE Verhalten</a> , markieren wir einen Artikel über Aggression, die eine natürliche Reaktion auf Eindringlinge in nahezu allen Tieren ist. <a href="http://www.jove.com/video/4367" target="_blank">Koolhaas, et al.</a> zeigen die resident-Eindringling Paradigma in Ratten und veranschaulichen verschiedene natürliche Verhaltensweisen gegen Eindringlinge, einschließlich der sozialen Exploration, seitliche Bedrohung, Verfolgung und Angriff. In <a href="http://www.jove.com/ctm" target="_blank">JoVE Clinical and Translational Medicine</a> , wir auf die Psoriasis ist eine chronische entzündliche Hauterkrankung konzentrieren. <a href="http://www.jove.com/video/50509" target="_blank">Gupta, et al.</a> zeigen die Goeckerman Regime, eine Psoriasis-Therapie, die UV-B (UVB) Phototherapie von rohem Kohlenteer (CCT) Gefolgt gilt. Entwickelt im Jahr 1925 von dem amerikanischen Dermatologen William H. Goeckerman, hat diese Behandlung ein ausgezeichnetes Sicherheitsprofil im Vergleich zu Immunsystem unterdrückende Medikamente, und ist äußerst wirksam bei der Behandlung von Psoriasis. Schließlich in <a href="http://www.jove.com/bioeng" target="_blank">JoVE Bioengineering</a> , markieren wir die unglaubliche Vielseitigkeit von Nukleinsäuren. Neben Speichern von Informationen, die unzählige biologische Prozesse leitet, können Nukleinsäuren programmiert in zweidimensionalen und dreidimensionalen Strukturen Selbstorganisation werden. <a href="http://www.jove.com/video/50268" target="_blank">Ben-Ishay, et al.</a> verwenden caDNAno Software zur DNA-Origami nanorobots Design, das können biologische Signale zu erfassen und spezifische Effekte ausüben. Diese nanoroBots nutzen die bemerkenswerten Eigenschaften von DNA und kann den Bereich der Nanotechnologie mit einer breiten Palette an möglichen therapeutischen und industrielle Anwendungen voranzutreiben. Sie hatten gerade einen kleinen Vorgeschmack von ein paar Highlights aus dem <a href="http://www.jove.com/archive/77" target="_blank">Juli 2013 Ausgabe</a> . Besuchen Sie die Website, um die Artikel in voller Länge und viele mehr in sehen <a href="http://www.jove.com/" target="_blank">JoVE: The Journal of Visualized Experiments.</a>

Watch this Scientific Journal Video about July 2013: This Month in JoVE at JoVE.com

July 2013: This Month in JoVE

Hier sind einige Highlights aus dem Juli 2013 Ausgabe des Journal of Visualized Experiments (JoVE).

Juli 2013: In diesem Monat in JoVE

Here's a look at what's coming up in the July 2013 issue of JoVE: The Journal of Visualized Experiments.
This month, we take a dip in the zebrafish tank ...

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7.0K Aufrufe.  Massachusetts Eye and Ear. Hier sind einige Highlights aus dem Juli 2013 Ausgabe des Journal of Visualized Experiments (JoVE).

Serie: July 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

August 2012: Diesen Monat in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Forschung

JoVE Journal

Biologie

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Das Jahr im Rückblick

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

Methods Article

Juli 2013: In diesem Monat in JoVE