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A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

DOI :

10.3791/55310-v

17:39 min

July 16th, 2017

July 16th, 2017

18,680 Views

1Department of Agronomy and Great Lakes Bioenergy Research Center, University of Wisconsin - Madison, 2Department of Soil Science, University of Wisconsin - Madison, 3Department of Soil, Water, and Climate, University of Minnesota, 4Faculty of Science and Engineering, Curtin University

The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.

Tags

Lipid Extraction

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