Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Traditional Chinese herbal medicine plays a role as an alternative method in treating many diseases, such as postmenopausal osteoporosis (POP). Gushukang ...

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

The human Fibroblast Growth Factor Receptor (FGFR) family comprises four members, namely, FGFR1, FGFR2, FGFR3, and FGFR4, that are involved in various ...

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

We describe the step-by-step procedure for culturing and differentiating mouse embryonic stem cells into neuronal lineages, followed by a series of assays ...

Shanghai University of Traditional Chinese Medicine

Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education

Guangdong Provincial Key Laboratory of Bioengineering Medicine

Penn State Hershey Cancer Institute

Background. Traditional Chinese herbal medicine (TCHM) is widely used for advanced gastric cancer (AGC) in China. In this study, the authors analyzed the prognostic factors of selected patients with AGC, and further studied the efficacy of TCHM (a herbal formula for invigorating spleen, formerly named Wei Chang' An) on AGC. METHODS: Patients with uncured AGC were prospectively enrolled. All patients were enrolled to either the TCHM group or non-TCHM group. TCHM was administered orally to the patients in the TCHM group for 3 months or more. Cox regression analysis was performed to determine survival trends adjusted for clinical and demographic factors. Kaplan-Meier curves were used to assess the differences in survival time. RESULTS: There were a total of 399 eligible patients with histologically confirmed adenocarcinoma of the stomach from 2001 to 2009. In the overall group, Cox regression analysis suggested that histological type (P = .016), radiotherapy (P = .000), cycle of chemotherapy (P = .000), and TCHM (P = .000) were independent prognostic factors. In a stratification analysis of stage for 213 patients who received 3 or more cycles of chemotherapy, there was a significant increase in median overall survival from 14.0 (non-TCHM group) to 20.0 (TCHM group) months (hazard ratio [HR] = 0.538, 95% confidence interval [CI] 0.385-0.750, P = .000). Among 186 patients who did not receive chemotherapy, but best supportive care, there was a significant increase in median overall survival from 7.0 (non-TCHM group) to 14.8 (TCHM group) months (HR = 0.443, 95% CI = 0.299-0.657, P = .000). CONCLUSIONS: TCHM has an important potential value for improving the prognosis of patients with AGC.

Survival Benefit of Traditional Chinese Herbal Medicine (a Herbal Formula for Invigorating Spleen) for Patients With Advanced Gastric Cancer.

Arri&egrave;re-plan. M&eacute;decine traditionnelle chinoise (TCHM) est largement utilis&eacute;e pour advanced cancer gastrique (AGC) en Chine. Dans cette &eacute;tude, les auteurs ont analys&eacute; les facteurs pronostiques des patients s&eacute;lectionn&eacute;s avec l&#39;AGC et poursuivi l&#39;&eacute;tude de l&#39;efficacit&eacute; de TCHM (une formule &agrave; base de plantes pour rate vivifiant, anciennement nomm&eacute; Wei Chang&#39; une) sur AGC. M&eacute;thodes : Patients avec AGC non durci ont suivi prospectivement. Tous les patients ont &eacute;t&eacute; inscrits le TCHM group ou groupe non-TCHM. TCHM a &eacute;t&eacute; administr&eacute; par voie orale &agrave; des patients dans le groupe TCHM pendant 3 mois ou plus. Analyse de r&eacute;gression de Cox a &eacute;t&eacute; r&eacute;alis&eacute;e pour d&eacute;terminer les tendances de survie ajust&eacute;es pour les facteurs d&eacute;mographiques et cliniques. Courbes de Kaplan-Meier ont &eacute;t&eacute; utilis&eacute;es pour &eacute;valuer les diff&eacute;rences de temps de survie. R&Eacute;SULTATS : Il y avait un total de 399 patients admissibles d&#39;un ad&eacute;nocarcinome histologiquement confirm&eacute; de l&#39;estomac de 2001 &agrave; 2009. Dans l&#39;ensemble du groupe, analyse de r&eacute;gression de Cox a propos&eacute; ce type histologique (P =.016), radioth&eacute;rapie (P = 000), cycle de chimioth&eacute;rapie (P = 000), et TCHM (P = 000) &eacute;taient des facteurs pronostiques ind&eacute;pendants. Dans une analyse de la stratification de la sc&egrave;ne pour 213 patients qui ont re&ccedil;u 3 ou plusieurs cycles de chimioth&eacute;rapie, il y avait une augmentation significative de la survie globale m&eacute;diane de 14,0 (groupe non-TCHM) &agrave; 20,0 (groupe TCHM) mois (risque relatif [HR] = 0,538, 95 % intervalle de confiance [IC] 0,385-0.750, P = 000. Parmi les 186 patients qui n&#39;ont pas re&ccedil;u de chimioth&eacute;rapie, mais les meilleurs soins de soutien, il y avait une augmentation significative de la survie globale m&eacute;diane de 7,0 (groupe non-TCHM) &agrave; 14,8 mois (groupe TCHM) (HR = 0,443, IC 95 % = 0,299-0,657, P = 000. CONCLUSIONS : TCHM a une valeur potentielle importante pour am&eacute;liorer le pronostic des patients avec l&#39;AGC.

B&eacute;n&eacute;fice en survie de m&eacute;decine chinoise traditionnelle (une formule &agrave; base de plantes pour vivifiant rate) pour les Patients atteints de Cancer gastrique avanc&eacute;.

Sullo sfondo. Medicina di erbe cinese tradizionale (TCHM) &egrave; ampiamente usato per advanced gastric cancer (AGC) in Cina. In questo studio, gli autori analizzati i fattori prognostici di pazienti selezionati con AGC e studiato ulteriormente l&#39;efficacia del TCHM (una formula a base di erbe per la milza tonificante, precedentemente denominato Chang Wei&#39; un) su AGC. Metodi: Pazienti con AGC non polimerizzato prospetticamente sono stati arruolati. Tutti i pazienti sono stati arruolati per il gruppo TCHM o gruppo non-TCHM. TCHM &egrave; stato somministrato per via orale per i pazienti del gruppo TCHM per 3 mesi o pi&ugrave;. Analisi di regressione di Cox &egrave; stata eseguita per determinare le tendenze di sopravvivenza aggiustate per fattori clinici e demografici. Curve di Kaplan-Meier sono state utilizzate per valutare le differenze nel tempo di sopravvivenza. RISULTATI: Ci sono stati un totale di 399 pazienti eleggibili con istologicamente confermato adenocarcinoma dello stomaco dal 2001 al 2009. Nel gruppo generale, analisi di regressione di Cox ha suggerito che tipo istologico (P =.016 dai radioterapia (P =.000 dai ciclo di chemioterapia (P =.000 dai e TCHM (P =.000) sono stati fattori prognostici indipendenti. In un&#39;analisi di stratificazione di fase per 213 pazienti che hanno ricevuto 3 o pi&ugrave; cicli di chemioterapia, ci era un aumento significativo nella sopravvivenza mediana da 14,0 (gruppo di non-TCHM) a 20,0 mesi (gruppo TCHM) (rapporto di rischio [HR] = 0.538, 95% intervallo di confidenza [CI] 0.385-0.750, P =.000 dentellatura Tra 186 pazienti che non hanno ricevuto chemioterapia, ma la miglior cura di supporto, c&#39;&egrave; stato un aumento significativo nella sopravvivenza mediana da 7.0 (gruppo di non-TCHM) a 14,8 mesi (gruppo TCHM) (HR = 0.443, 95% CI = 0.299-0.657, P =.000 dentellatura CONCLUSIONI: TCHM ha un importante valore potenziale per migliorare la prognosi dei pazienti con AGC.

Beneficio di sopravvivenza della medicina tradizionale cinese a base di erbe (una Formula a base di erbe per tonificante della milza) per i pazienti con cancro gastrico avanzato.

Neovascularization and expression of inflammatory cytokines were examined in Osteoprotegerin (Opg) knockout (KO) mice that show intervertebral disc (IVD) degeneration.

Deletion of Opg Leads to Increased Neovascularization and Expression of Inflammatory Cytokines in the Lumbar Intervertebral Disc of Mice.

Ligusticum chuanxiong (LC)-Gastrodia elata (GE) compatibility is widely used in the clinic for the treatment of migraine. It has been shown that the changes of neurotransmitters in the central nervous system are closely related to the pathogenesis of migraine; whether LC-GE compatibility might affect the neurotransmitters in migraine rats has not yet been studied. In this study, high performance liquid chromatography-fluorescence detector methods for quantification of serotonin (5-hydroxytryptamine, 5-HT) and excitatory amino acids (EAAs) in rat brain were developed. The 5-HT was measured directly, while EAAs were determined by using dansyl chloride as precolumn derivative reagent. The validation of the methods, including selectivity, linearity, sensitivity, precision, accuracy, recoveries, and stability were carried out and demonstrated to meet the requirements of quantitative analysis. Compared with the model group, the expression of 5-HT in migraine rat brain was enhanced from 30 minutes to 120 minutes and glutamate (L-Glu) was suppressed from 30 minutes to 60 minutes in an LC-GE (4:3) group compared with the model group (p < 0.05, p < 0.01, respectively). These findings showed that the analytical methods were simple, sensitive, selective, and low cost, and LC-GE 4:3 compatibility could have better efficacy for treating migraine through upregulating 5-HT levels and downregulating L-Glu levels.

Determination of the levels of two types of neurotransmitter and the anti-migraine effects of different dose-ratios of Ligusticum chuanxiong and Gastrodia elata.

Primary osteoporosis (POP), which is caused by unbalanced bone remodeling, leads to significant economic and societal burdens globally. Gushukang (GSK) granule serves as one commonly used prescription for POP in Traditional Chinese Medicine (TCM). The present study aimed to clarify the exact roles of GSK in bone remodeling with in vivo and in vitro assays. Here we showed that GSK prevented bone loss and the alternations of osteoporotic bone parameters as well as the decreased density of osteoclast in ovariectomized (OVX) mice. GSK inhibited receptor activator for nuclear factor-κ B Ligand (RANKL)-activated osteoclastogenesis in bone marrow macrophages (BMMs). At the molecular levels, GSK inhibited the expression of nuclear factor of activated T cells cytoplasm 1(NFATc1) and c-Fos, two master regulators of osteoclastogenesis. GSK also inhibited bone resorbed genetic expression of matrix metalloproteinase 9 (MMP9), cathepsin K (Ctsk), TRAP and carbonic anhydrase II (Car2). Meanwhile, GSK stimulated osteoblastogenesis from bone primary mesenchymal stem cells (MSCs) and enhanced the expression of Osteirx, and Runx2. GSK also stimulated the expression of Col-1, Osteocalcein and alkaline phosphatase (ALP). Our investigation established the systemic bone protective effects of GSK by suppressing osteoclastogenesis and stimulating osteoblastogenesis and laid bases for new drugs discovery in treating POP.

The systemic bone protective effects of Gushukang granules in ovariectomized mice by inhibiting osteoclastogenesis and stimulating osteoblastogenesis.

This study was to determine the bone protective effects and underlying mechanisms of Osthole (OT) in ovariectomized (OVX) mice. We found that the inhibitory effects of OT on receptor activator of nuclear factor kappa-B ligand (RANKL)-activated osteoclastogenesis are responsible for its bone protective effects in OVX mice.

The naturally derived small compound Osthole inhibits osteoclastogenesis to prevent ovariectomy-induced bone loss in mice.

Recent studies on Polycomb repressive complexes (PRC) reveal a surprising role in transcriptional activation, yet the underlying mechanism remains poorly understood. We previously identified a type 1 PRC (PRC1) that contains Autism Susceptibility Candidate 2 (AUTS2), which positively regulates transcription of neuronal genes. However, the mechanism by which the PRC1-AUTS2 complex influences neurodevelopment is unclear. Here we demonstrate that WDR68 is not only an integral component of the PRC1-AUTS2 complex, but it is also required for PRC1-AUTS2-mediated transcription activation. Furthermore, deletion of Wdr68 in mouse embryonic stem cells leads to defects in neuronal differentiation without affecting self-renewal. Through transcriptomic analysis, we found that many genes responsible for neuronal differentiation are down-regulated in Wdr68 deficient neural progenitors. These genes include those targeted by the PRC1-AUTS2 complex. In summary, our studies uncovered a previously unknown but essential component of the active PRC1 complex and evidence of its role in regulating the expression of genes that are important for neuronal differentiation.

Qiang Wang

Longhua Hospital,
Spine Institute

Shanghai University of Traditional Chinese Medicine

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Qiang Wang

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Longhua Hospital,Spine Institute

Shanghai University of Traditional Chinese Medicine

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Longhua Hospital,
Spine Institute