University Hospital
2 ARTICLES PUBLISHED IN JoVE
Biology
Simple Detection of Primary Cilia by Immunofluorescence
Alzbeta Filipova 1, Daniel Diaz Garcia 2, Josef Dvorak 3, Stanislav Filip 4, Marcela Jelicova 1, Zuzana Sinkorova 1
1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University
Primary cilia are extracellular structures associated with the centriole. Primary cilia detection by immunofluorescent staining is a relatively simple procedure that results in extremely high-quality images. In this protocol, fibroblasts expressing primary cilia were fixed, immunostained, and imaged in a fluorescent or confocal microscope.
Neuroscience
Quantified Assessment of Infant's Gross Motor Abilities Using a Multisensor Wearable
Elisa Taylor 1, Manu Airaksinen 1, Anastasia Gallen 1, Tuuli Immonen 2, Elina Ilén 3, Taru Palsa 1,2, Leena M. Haataja 1,2, Sampsa Vanhatalo 1,4
1BABA Center, Pediatric Research Center, Department of Clinical Neurophysiology, New Children's Hospital and HUS Imaging, Helsinki, University Hospital, 2Department of Pediatric Neurology, Children's Hospital, Helsinki University Hospital and University of Helsinki, 3Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, 4Department of Physiology, University of Helsinki
This paper outlines the assessment of infants' gross motor performance with a multisensor wearable and its fully automated deep learning-based analysis pipeline. The method quantifies the posture and movement patterns of infants from lying supine until they master walking independently.
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