Accedi

University Hospital

2 ARTICLES PUBLISHED IN JoVE

Biology

Simple Detection of Primary Cilia by Immunofluorescence

Alzbeta Filipova¹, Daniel Diaz Garcia², Josef Dvorak³, Stanislav Filip⁴, Marcela Jelicova¹, Zuzana Sinkorova¹

¹Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, ²Department of Clinical Biochemistry and Diagnostics, University Hospital, ³Department of Oncology, Thomayer Hospital, Charles University, ⁴Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

Primary cilia are extracellular structures associated with the centriole. Primary cilia detection by immunofluorescent staining is a relatively simple procedure that results in extremely high-quality images. In this protocol, fibroblasts expressing primary cilia were fixed, immunostained, and imaged in a fluorescent or confocal microscope.

Neuroscience

Quantified Assessment of Infant's Gross Motor Abilities Using a Multisensor Wearable

Elisa Taylor¹, Manu Airaksinen¹, Anastasia Gallen¹, Tuuli Immonen², Elina Ilén³, Taru Palsa^1,2, Leena M. Haataja^1,2, Sampsa Vanhatalo^1,4

¹BABA Center, Pediatric Research Center, Department of Clinical Neurophysiology, New Children's Hospital and HUS Imaging, Helsinki, University Hospital, ²Department of Pediatric Neurology, Children's Hospital, Helsinki University Hospital and University of Helsinki, ³Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, ⁴Department of Physiology, University of Helsinki

This paper outlines the assessment of infants' gross motor performance with a multisensor wearable and its fully automated deep learning-based analysis pipeline. The method quantifies the posture and movement patterns of infants from lying supine until they master walking independently.