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Wistar Institute

3 ARTICLES PUBLISHED IN JoVE

Medicine

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Melanie R. Rutkowski^*1, Michael J. Allegrezza^*1, Nikolaos Svoronos¹, Amelia J. Tesone¹, Tom L. Stephen¹, Alfredo Perales-Puchalt¹, Jenny Nguyen¹, Paul J. Zhang², Steven N. Fiering³, Julia Tchou^4,5,6, Jose R. Conejo-Garcia¹

¹Tumor Microenvironment and Metastasis Program, Wistar Institute, ²Department of Pathology and Lab Medicine, Perelman School of Medicine, University of Pennsylvania, ³Department of Microbiology and Immunology and Department of Genetics, Geisel School of Medicine at Dartmouth, ⁴Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine, University of Pennsylvania, ⁵Rena Rowan Breast Center, Abramson Cancer Center, University of Pennsylvania, ⁶Center for Advanced Medicine, University of Pennsylvania

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

Cancer Research

A Melanoma Patient-Derived Xenograft Model

Min Xiao¹, Vito W. Rebecca¹, Meenhard Herlyn¹

¹Wistar Institute

Patient-derived xenograft (PDX) models more robustly recapitulate melanoma molecular and biological features and are more predictive of therapy response compared to traditional plastic tissue culture-based assays. Here we describe our standard operating protocol for the establishment of new PDX models and the characterization/experimentation of existing PDX models.

Immunology and Infection

Gene Editing of Primary Rhesus Macaque B Cells

Harald Hartweger¹, Rajeev Gautam², Yoshiaki Nishimura², Fabian Schmidt^3,5, Kai-Hui Yao¹, Amelia Escolano^1,6, Mila Jankovic¹, Malcolm A. Martin², Michel C. Nussenzweig^1,4

¹Laboratory of Molecular Immunology, The Rockefeller University, ²Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, ³Laboratory of Retrovirology, The Rockefeller University, ⁴Howard Hughes Medical Institute, The Rockefeller University, ⁵Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), ⁶Vaccine and Immunotherapy Center, Wistar Institute

We present a method for culturing and gene editing primary rhesus macaque B cells using CRISPR/Cas9 and recombinant adeno-associated virus serotype 6 for the study of B cell therapies.