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Max Planck Institute for Biophysical Chemistry

5 ARTICLES PUBLISHED IN JoVE

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Biology

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
Mariya M. Kucherenko 1, April K. Marrone 1, Valentyna M. Rishko 1, Andriy S. Yatsenko 1, Annekatrin Klepzig 1, Halyna R. Shcherbata 1
1Gene Expression and Signaling Research Group, Max Planck Institute for Biophysical Chemistry

Identification of mechanisms underlying muscle damage is crucial. Here we present the histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. This allows analysis of muscle morphology and localization of protein and other muscle cell components.

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Biology

Measurement of Metabolic Rate in Drosophila using Respirometry
Andriy S. Yatsenko 1, April K. Marrone 1, Mariya M. Kucherenko 1, Halyna R. Shcherbata 1
1Max Planck Research Group of Gene Expression and Signaling, Max Planck Institute for Biophysical Chemistry

Metabolic disorders are among one of the most common diseases in humans. The genetically tractable model organism D. melanogaster can be used to identify novel genes that regulate metabolism. This paper describes a relatively simple method which allows studying the metabolic rate in flies by measuring their CO2 production.

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Medicine

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Hanibal Bohnenberger 1, Philipp Ströbel 1, Sebastian Mohr 2, Jasmin Corso 3, Tobias Berg 2, Henning Urlaub 3,4, Christof Lenz 3,4, Hubert Serve 2,5,6, Thomas Oellerich 2,5,6
1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center

In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.

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Neuroscience

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
Michal Turek 1, Judith Besseling 1, Henrik Bringmann 1
1Max Planck Institute for Biophysical Chemistry

Imaging behavior and neural activity over long time scales without immobilization of the animal is a prerequisite to understand behavior. Agarose microfluidic chambers imaging (AMI) can be used to image neural activity and behavior for all life stages of Caenorhabditis elegans.

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Medicine

A Model of Reverse Vascular Remodeling in Pulmonary Hypertension Due to Left Heart Disease by Aortic Debanding in Rats
Pengchao Sang 1,2,3, Mariya M. Kucherenko 1,2,3, Juquan Yao 2, Qiuhua Li 2, Szandor Simmons 2,3, Wolfgang M. Kuebler *2,3, Christoph Knosalla *1,3,4
1Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin (DHZB), 2Institute of Physiology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, 4Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

The present protocol describes a surgical procedure to remove ascending-aortic banding in a rat model of pulmonary hypertension due to left heart disease. This technique studies endogenous mechanisms of reverse remodeling in the pulmonary circulation and the right heart, thus informing strategies to reverse pulmonary hypertension and/or right ventricular dysfunction.

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