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Hanyang University

8 ARTICLES PUBLISHED IN JoVE

Engineering

Testing of Nanoparticle Release from a Composite Containing Nanomaterial Using a Chamber System

¹Department of Mechanical Engineering, Hanyang University, ²Institute of Nanoproduct Safety Research, Hoseo University

Nanoparticle release is tested using a chamber system that includes a condensation particle counter, an optical particle counter and sampling ports to collect filter samples for microscopy analysis. The proposed chamber system can be effectively used for nanomaterial release testing with a repeatable and consistent data range.

Biochemistry

Oligopeptide Competition Assay for Phosphorylation Site Determination

Min Sung Joo¹, Ja Hyun Koo¹, Sol-Bi Shin², Hyungshin Yim², Sang Geon Kim¹

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

Peptide competition assays are widely used in a variety of molecular and immunological experiments. This paper describes a detailed method for an in vitro oligopeptide-competing kinase assay and the associated validation procedures, which may be useful to find specific phosphorylation sites.

Chemistry

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Byung Hyo Kim^1,2, Junyoung Heo^1,2, Won Chul Lee³, Jungwon Park^1,2

¹Center for Nanoparticle Research, Institute for Basic Science (IBS), ²School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, ³Department of Mechanical Engineering, Hanyang University

Here we introduce experimental protocols for the real-time observation of a self-assembly process using liquid-cell transmission electron microscopy.

Behavior

Measuring the Kinematics of Daily Living Movements with Motion Capture Systems in Virtual Reality

Kyoungwon Seo¹, Ahreum Lee¹, Jieun Kim², Hokyoung Ryu³, Hojin Choi⁴

¹Department of Industrial Engineering, Hanyang University, ²Imagine Lab, Hanyang University, ³Department of Arts & Technology, Hanyang University, ⁴Department of Neurology, College of Medicine, Hanyang University

We designed a virtual reality test to assess instrumental activities of daily living (IADL) with a motion capture system. We propose a detailed kinematic analysis to interpret the participant's various movements, including trajectory, moving distance, and time to completion to evaluate IADL capabilities.

Chemistry

Solution-Processed "Silver-Bismuth-Iodine" Ternary Thin Films for Lead-Free Photovoltaic Absorbers

Jae Taek Oh^1,2, Dae-Hwan Kim¹, Younghoon Kim¹

¹Convergence Research Center for Solar Energy, Daegu Gyeongbuk Institute of Science and Technology (DGIST), ²Department of Chemistry and Research Institute for Convergence of Basic Sciences, Hanyang University

Herein, we present detailed protocols for solution-processed silver-bismuth-iodine (Ag-Bi-I) ternary semiconductor thin films fabricated on TiO₂-coated transparent electrodes and their potential application as air-stable and lead-free optoelectronic devices.

Immunology and Infection

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

Irfan Ullah^1,2, Kunho Chung¹, Jagadish Beloor², Sang-Kyung Lee¹, Priti Kumar²

¹Department of Bioengineering and Institute of Nanoscience and Technology, Hanyang University, ²Department of Internal Medicine, Section of Infectious Diseases, Yale University School of Medicine

Here, we present a protocol using a mouse positioning device that enables the appropriate placement of mice for the intranasal administration of a brain-targeting peptide-siRNA formulation enabling effective gene silencing in the central nervous system.

Cancer Research

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Moe Ishihara^*1, Junhui Hu^*1, Xiaoyu Zhang², YongHyeon Choi³, Anthony Wong⁴, Celine Cano-Ruiz⁵, Rongwei Zhao⁶, Ping Tan⁷, Jonathan L. Tso¹, Lily Wu^1,8

¹Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, ²Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, ³Department of Bioengineering, Hanyang University, ⁴Department of Ecology and Evolutionary Biology, University of California, Los Angeles, ⁵Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, ⁶School of Life Sciences, Beijing Normal University, ⁷Department of Urology, West China Hospital, Sichuan University, ⁸Department of Urology, David Geffen School of Medicine, University of California, Los Angeles

Metastatic clear cell renal cell carcinoma is a disease without a comprehensive animal model for thorough preclinical investigation. This protocol illustrates two novel animal models for the disease: the orthotopically implanted mouse model and the chicken chorioallantoic membrane model, both of which demonstrate lung metastasis resembling clinical cases.

Chemistry

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Sophie M. Briffa¹, Jo Sullivan², Agnieszka Siupa², Pauline Carnell-Morris², Michele Carboni², Kerstin Jurkschat³, Ruud J. B. Peters⁴, Carolin Schultz⁵, Kang Hee Seol⁶, Sook-Jin Kwon⁶, Sehee Park⁷, Tae Hyun Yoon^6,7, Colin Johnston³, Stephen Lofts⁸, Eugenia Valsami-Jones¹

¹School of Geography, Earth and Environmental Sciences, University of Birmingham, ²Malvern Panalytical, ³Department of Materials, University of Oxford, ⁴Wageningen Food Safety Research, ⁵UK Centre for Ecology & Hydrology, ⁶Institute for Next Generation Material Design, Hanyang University, ⁷Department of Chemistry, College of Natural Sciences, Hanyang University, ⁸UK Centre for Ecology & Hydrology

The protocol described here aims to measure the hydrodynamic diameter of spherical nanoparticles, more specifically gold nanoparticles, in aqueous media by means of Nanoparticle Tracking Analysis (NTA). The latter involves tracking the movement of particles due to Brownian motion and implementing the Stokes-Einstein equation to obtain the hydrodynamic diameter.