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University of Maribor

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
Marko Milojević 1, Boštjan Vihar 1,2, Luka Banović 2, Mihael Miško 2, Lidija Gradišnik 1, Tanja Zidarič 1, Uroš Maver 1,3
1Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, 2Institute for Development of Advanced Applied Systems (IRNAS), 3Department of Pharmacology, Faculty of Medicine, University of Maribor

Presented here is a simple-to-use, core/shell, three-dimensional bioprinting set-up for one-step fabrication of hollow scaffolds, suitable for tissue engineering of vascular and other tubular structures.

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Biology

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
Andraž Stožer 1, Jurij Dolenšek 1,2, Lidija Križančić Bombek 1, Viljem Pohorec 1, Marjan Slak Rupnik 1,3, Maša Skelin Klemen 1
1Institute of Physiology, Faculty of Medicine, University of Maribor, 2Faculty of Natural Sciences and Mathematics, University of Maribor, 3Center for Physiology and Pharmacology, Medical University of Vienna

We present the preparation of acute pancreatic tissue slices and their use in confocal laser scanning microscopy to study calcium dynamics simultaneously in a large number of live cells, over long time periods, and with high spatiotemporal resolution.

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Chemistry

Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS
Matjaž Rantaša 1, David Majer 1, Matjaž Finšgar 1
1Faculty of Chemistry and Chemical Engineering, University of Maribor

The presented protocol describes sample homogenization with a laboratory mixer, acid digestion of food samples using a mixture of 68 wt% HNO3 and 30 wt% H2O2 via microwave-assisted wet acid digestion, and multi-element determination performed with inductively coupled plasma mass spectrometry.

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