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Georgia Institute of Technology and Emory University

7 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Douglas Nam¹, Chih-Wen Ni², Amir Rezvan¹, Jin Suo², Klaudia Budzyn¹, Alexander Llanos¹, David G. Harrison¹, Don P. Giddens², Hanjoong Jo^1,2,3

¹Department of Medicine, Division of Cardiology, Emory University, ²Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, ³Department of Bioinspired Science, Ewha Womans University

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

David R. Myers^*1,2,3,4, Yumiko Sakurai^*1,2,3,4, Reginald Tran^1,2,3,4, Byungwook Ahn^1,2,3,4, Elaissa Trybus Hardy^1,2,3,4, Robert Mannino^1,2,3,4, Ashley Kita^1,2,3,4, Michelle Tsai^1,2,3,4, Wilbur A. Lam^1,2,3,4

¹Department of Pediatrics, Emory University School of Medicine, ²Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ³Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta , ⁴Winship Cancer Institute of Emory University

A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.

Medicine

Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography

Sandeep Kumar¹, Zachary Berriochoa¹, Alex D. Jones¹, Yingbin Fu^1,2

¹Department of Ophthalmology & Visual Sciences, University of Utah Health Sciences Center, ²Department of Neurobiology & Anatomy, University of Utah Health Sciences Center

Indocyanine Green Angiography (or ICGA) performed by tail vein injection provides high quality ICGA time course images to characterize abnormalities in mouse choroid.

Neuroscience

Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury

Rowan O. Brothers^*1, Sara Bitarafan^*2,3, Alyssa F. Pybus^1,3, Levi B. Wood^*1,2,3, Erin M. Buckley^*1,4,5

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, ³Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, ⁴Department of Pediatrics, Emory University School of Medicine, ⁵Children's Research Scholar, Children's Healthcare of Atlanta

This protocol presents methods to characterize the neuroinflammatory and hemodynamic response to mild traumatic brain injury and to integrate these data as part of a multivariate systems analysis using partial least squares regression.

Bioengineering

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Rong Ma¹, Anna V. Kellner², Yuesong Hu¹, Brendan R. Deal¹, Aaron T. Blanchard², Khalid Salaita^1,2

¹Department of Chemistry, Emory University, ²Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

This paper describes a detailed protocol for using DNA-based tension probes to image the receptor forces applied by immune cells. This approach can map receptor forces >4.7pN in real-time and can integrate forces over time.

Bioengineering

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets

Andrew Zhao¹, Jeungyoon Lee², Stanislav Emelianov^1,3

¹Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²School of Electrical Engineering, Georgia Institute of Technology, ³Department of Biomedical Engineering and School of Electrical Engineering, Georgia Institute of Technology and Emory University

Optically activated perfluorocarbon nanodroplets show promise in imaging applications outside of the vascular system. This article will demonstrate how to synthesize these particles, crosslink polyacrylamide phantoms, and modulate the droplets acoustically to enhance their signal.

Biology

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Sandeep Kumar^*1, Aitor Andueza^*1, Nicolas Villa-Roel¹, Juyoung Kim¹, Dong-Won Kang¹, Hanjoong Jo^1,2

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²Division of Cardiology, Georgia Institute of Technology and Emory University

We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.