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University of Maryland College Park

3 ARTICLES PUBLISHED IN JoVE

Biology

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

Chloe Mara¹, Boyana Grigorova¹, Zhongchi Liu¹

¹Department of Cell Biology and Molecular Genetics, University of Maryland College Park

This article illustrates the floral-dip method of Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana. By introducing a cell-cycle regulated promoter-reporter, pTSO2::β-glucuronidase (GUS), into Arabidopsis, we illustrates how one detects GUS reporter expression in transgenic seedlings.

Biology

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Courtney A. Hollender¹, Zhongchi Liu¹

¹Dept. Of Cell Biology and Molecular Genetics, University of Maryland

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Biology

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Ying Wu^*1, Darwin Diaz^*1, Jian Yin¹, David Bloodgood¹, William Sexton¹, Cheng-I Wei², Shunyuan Xiao^1,3

¹Institute for Bioscience and Biotechnology Research, University of Maryland, ²Department of Nutrition and Food Science, University of Maryland College Park, ³Department of Plant Sciences and Landscape Architecture, University of Maryland College Park

We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.