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University of Maryland College Park

3 ARTICLES PUBLISHED IN JoVE

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Biology

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Chloe Mara 1, Boyana Grigorova 1, Zhongchi Liu 1
1Department of Cell Biology and Molecular Genetics, University of Maryland College Park

This article illustrates the floral-dip method of Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana. By introducing a cell-cycle regulated promoter-reporter, pTSO2::β-glucuronidase (GUS), into Arabidopsis, we illustrates how one detects GUS reporter expression in transgenic seedlings.

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Biology

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
Courtney A. Hollender 1, Zhongchi Liu 1
1Dept. Of Cell Biology and Molecular Genetics, University of Maryland

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

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Biology

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
Ying Wu *1, Darwin Diaz *1, Jian Yin 1, David Bloodgood 1, William Sexton 1, Cheng-I Wei 2, Shunyuan Xiao 1,3
1Institute for Bioscience and Biotechnology Research, University of Maryland, 2Department of Nutrition and Food Science, University of Maryland College Park, 3Department of Plant Sciences and Landscape Architecture, University of Maryland College Park

We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.

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