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Vanderbilt School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Joseph Dragavon¹, Carolyn Sinow², Alexandra D. Holland¹, Abdessalem Rekiki¹, Ioanna Theodorou³, Chelsea Samson⁴, Samantha Blazquez¹, Kelly L. Rogers⁵, Régis Tournebize^1,6,7, Spencer L. Shorte¹

¹Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, ²Department of Radiation Oncology, Stanford School of Medicine, ³Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, ⁴Vanderbilt School of Medicine, ⁵The Walter & Eliza Hall Institute of Medical Research, ⁶Unité INSERM U786, Institut Pasteur, ⁷Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur

Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.

Biology

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Rafeeh Dinani^1,2, Emmy Manders^1,2,3, Michiel Helmes^1,2,3, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster^1,2, Jolanda van der Velden^1,2

¹Division of Physiology, Amsterdam UMC, Vrije University, ²Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology, Vanderbilt School of Medicine

Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.