Vanderbilt School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
Joseph Dragavon 1, Carolyn Sinow 2, Alexandra D. Holland 1, Abdessalem Rekiki 1, Ioanna Theodorou 3, Chelsea Samson 4, Samantha Blazquez 1, Kelly L. Rogers 5, Régis Tournebize 1,6,7, Spencer L. Shorte 1
1Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, 2Department of Radiation Oncology, Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786, Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur
Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.
Biology
Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Rafeeh Dinani 1,2, Emmy Manders 1,2,3, Michiel Helmes 1,2,3, Lili Wang 4, Bjorn C. Knollmann 4, Diederik W. D. Kuster 1,2, Jolanda van der Velden 1,2
1Division of Physiology, Amsterdam UMC, Vrije University, 2Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, 3CytoCypher BV, 4Division of Clinical Pharmacology, Vanderbilt School of Medicine
Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.
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