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Shiv Nadar University

2 ARTICLES PUBLISHED IN JoVE

Environment

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Deepa Sethi¹, Richa Priyadarshini¹

¹Department of Life Sciences, School of Natural Sciences, Shiv Nadar University

We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.

Genetics

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Shalini Soni¹, Shubhra Agarwal¹, Rakesh Aggarwal², Naga Suresh Veerapu¹

¹Virology Section, Department of Life Sciences, Shiv Nadar University, ²Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences

The protocol describes a method for introducing controllable genetic diversity in the hepatitis C virus genome by combining full-length mutant RNA synthesis using error-prone PCR and reverse genetics. The method provides a model for phenotype selection and can be used for 10 kb long positive-sense RNA virus genomes.