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Northwestern Polytechnical University

5 ARTICLES PUBLISHED IN JoVE

Cancer Research

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Rui Xue Zhang¹, Tian Zhang¹, King Chen¹, Ji Cheng¹, Paris Lai¹, Andrew M. Rauth², K. Sandy Pang¹, Xiao Yu Wu¹

¹Department of Pharmaceutical Sciences, University of Toronto, ²Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

This protocol describes an efficient and convenient analytical process of sample extraction and simultaneous determination of multiple drugs, doxorubicin (DOX), mitomycin C (MMC) and a cardio-toxic DOX metabolite, doxorubicinol (DOXol), in the biological samples from a preclinical breast tumor model treated with nanoparticle formulations of synergistic drug combination.

Biochemistry

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

Xiaoying Qi^*1, Yunyun Zhang^*2, Yuan Zhang¹, Tianhui Ni³, Wenfeng Zhang², Chunhua Yang¹, Jia Mi^1,4, Jiandi Zhang^2,3, Geng Tian¹

¹Medicine and Pharmacy Research Center, Binzhou Medical University, ²Yantai Zestern Biotechnique Co. LTD, ³Precision Medicine research center, Binzhou Medical University, ⁴Department of Chemistry - BMC, Uppsala University

Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.

Medicine

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch

Yaguang Li¹, Junhong Liu^2,3, Gaixia Liu¹, Zhenhong Pan^2,3, Mingxia Zhang¹, Yao Ma⁴, Qingxia Wei^1,5, Hongping Xia^1,6, Rui Xue Zhang^1,2, Junjun She¹

¹Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, ²Institute of Medical Research, Northwestern Polytechnical University, ³School of Life Sciences, Northwestern Polytechnical University, ⁴Department of Medicine, Xi'an Jiaotong University, ⁵Princess Margaret Cancer Center, University of Health Network, ⁶Department of Pathology, Nanjing Medical University

The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.

Medicine

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

Weijia Lu^*1, Ruimin Miao^*1, Sijun Hu¹, Junhong Liu¹, Fanqi Jin¹, Rui Xue Zhang¹

¹Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University

Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress.

Immunology and Infection

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Yang Xie^*1, Yuan Zhang^*2, Yitian Liu³, Yuling Wang⁴, Kun Cheng⁴, Ran Zhuang⁴, Ka Bian¹

¹Otolaryngological Department of Tangdu Hospital, Fourth Military Medical University, ²Institute of Medical Research, Northwestern Polytechnical University, ³Orthopedic Department of Tangdu Hospital, Fourth Military Medical University, ⁴Department of Immunology, Fourth Military Medical University

Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.