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Singapore National Eye Centre

4 ARTICLES PUBLISHED IN JoVE

Medicine

A Step by Step Protocol for Subretinal Surgery in Rabbits

Sami Al-Nawaiseh¹, Fabian Thieltges¹, Zengping Liu^1,2, Claudine Strack¹, Ralf Brinken¹, Norbert Braun³, Marc Wolschendorf³, Arvydas Maminishkis⁵, Nicole Eter⁴, Boris V. Stanzel^1,6

¹Department of Ophthalmology, University of Bonn, ²Department of Ophthalmology, National University of Singapore, ³Geuder AG, ⁴Department of Ophthalmology, University of Münster, ⁵Section on Epithelial and Retinal Physiology and Disease, National Eye Institute/National Institutes of Health, ⁶Surgical Retina Department, Singapore National Eye Centre

Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.

Medicine

Using Retinal Imaging to Study Dementia

Victor T.T. Chan¹, Tiffany H.K. Tso¹, Fangyao Tang¹, Clement Tham¹, Vincent Mok^2,3,4, Christopher Chen^5,6, Tien Y. Wong^7,8, Carol Y. Cheung¹

¹Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, ²Department of Medicine & Therapeutics, The Chinese University of Hong Kong, ³Therese Pei Fong Chow Research Centre for Prevention of Dementia, The Chinese University of Hong Kong, ⁴Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, ⁵Memory Aging and Cognition Centre, National University Health System, ⁶Department of Pharmacology, National University of Singapore, ⁷Singapore Eye Research Institute, Singapore National Eye Centre, ⁸Duke-NUS Medical School, National University of Singapore

The retina shares prominent similarities with the brain and thus represents a unique window to study vasculature and neuronal structure in the brain non-invasively. This protocol describes a method to study dementia using retinal imaging techniques. This method can potentially aid in diagnosis and risk assessment of dementia.

Medicine

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Ivan Seah^*1,2, Zengping Liu^*1,3,4, Daniel Soo Lin Wong¹, Wendy Wong², Graham E. Holder^1,2,5, Veluchamy Amutha Barathi^1,4,6, Gopal Lingam^1,2,4, Xinyi Su^1,2,3,4, Boris V. Stanzel^1,7,8

¹Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, ²Department of Ophthalmology, National University Hospital, Singapore, ³Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), ⁴Singapore Eye Research Institute (SERI), ⁵UCL Institute of Ophthalmology, ⁶Academic Clinical Program in Ophthalmology, Duke-NUS Medical School, ⁷Macula Center Saar, Eye Clinic Sulzbach, Knappschaft Hospital Saar, ⁸Department of Ophthalmology, University of Bonn

The non-human primate (NHP) is an ideal model for studying human retinal cellular therapeutics due to the anatomical and genetic similarities. This manuscript describes a method for submacular transplantation of retinal pigment epithelial cells in the NHP eye and strategies to prevent intraoperative complications associated with macular manipulation.

Bioengineering

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Su Yin Chaw^1,2, Tina Tzee Ling Wong^3,4, Subbu Venkatraman^2,5, Ann-Marie Chacko¹

¹Laboratory for Translational and Molecular Imaging, Cancer and Stem Cell Biology Programme, Duke-NUS Medical School, ²School of Materials Science and Engineering, Nanyang Technological University, ³Singapore National Eye Centre, ⁴Singapore Eye Research Institute, ⁵Material Science & Engineering, National University of Singapore

We present a protocol for the use of fiberoptic confocal laser microendoscopy (CLM) to non-invasively study the spatio-temporal distribution of liposomes in the eye after subconjunctival injection.